Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   J8N01_RS12265 Genome accession   NZ_CP073301
Coordinates   1915309..1915731 (-) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium strain HT517     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1910309..1920731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8N01_RS12235 (J8N01_12100) - 1910425..1910910 (+) 486 WP_013057955.1 hypothetical protein -
  J8N01_RS12240 (J8N01_12105) - 1911210..1911755 (-) 546 WP_013057954.1 GNAT family N-acetyltransferase -
  J8N01_RS12245 (J8N01_12110) - 1912053..1913333 (+) 1281 WP_013057953.1 glycosyltransferase -
  J8N01_RS12250 (J8N01_12115) - 1913356..1914012 (+) 657 WP_013057952.1 TetR/AcrR family transcriptional regulator -
  J8N01_RS12255 (J8N01_12120) - 1914168..1914761 (+) 594 WP_013057951.1 hypothetical protein -
  J8N01_RS12260 (J8N01_12125) - 1914802..1915146 (-) 345 WP_013057950.1 DUF1516 family protein -
  J8N01_RS12265 (J8N01_12130) nucA/comI 1915309..1915731 (-) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  J8N01_RS12270 - 1915872..1916054 (-) 183 WP_274496375.1 hypothetical protein -
  J8N01_RS12275 - 1916075..1916335 (-) 261 WP_274496376.1 hypothetical protein -
  J8N01_RS12280 (J8N01_12140) - 1916365..1917747 (-) 1383 WP_013057947.1 PepSY-associated TM helix domain-containing protein -
  J8N01_RS12285 (J8N01_12145) - 1917860..1918852 (-) 993 WP_013057946.1 glycerophosphodiester phosphodiesterase family protein -
  J8N01_RS12290 (J8N01_12150) - 1919028..1919669 (-) 642 WP_013057945.1 response regulator -
  J8N01_RS12295 (J8N01_12155) - 1919670..1920491 (-) 822 WP_029322903.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=558914 J8N01_RS12265 WP_013057949.1 1915309..1915731(-) (nucA/comI) [Priestia megaterium strain HT517]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=558914 J8N01_RS12265 WP_013057949.1 1915309..1915731(-) (nucA/comI) [Priestia megaterium strain HT517]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGGGCTGAGGAAAACCGCCGTGAGTCTTTAAAA
GGCATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGTGCCGATAT
TGAATATGTAAAGCCTTCAGACAATCGAGGCGCCGGGTCTTGGATCTCACATCAAGTAGACGAGTTTCCTGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507