Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   J8N01_RS09085 Genome accession   NZ_CP073301
Coordinates   1379812..1380186 (-) Length   124 a.a.
NCBI ID   WP_223269005.1    Uniprot ID   -
Organism   Priestia megaterium strain HT517     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1374812..1385186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8N01_RS09060 (J8N01_08960) - 1375386..1375754 (-) 369 WP_013058566.1 winged helix-turn-helix transcriptional regulator -
  J8N01_RS09065 (J8N01_08965) - 1375994..1376512 (+) 519 WP_235565579.1 enoyl-CoA hydratase/isomerase family protein -
  J8N01_RS09070 - 1376652..1376777 (+) 126 WP_016765178.1 hypothetical protein -
  J8N01_RS09075 (J8N01_08970) helD 1377022..1379340 (+) 2319 WP_013058564.1 RNA polymerase recycling motor HelD -
  J8N01_RS09080 (J8N01_08975) comJ 1379369..1379776 (-) 408 WP_013058563.1 competence protein ComJ -
  J8N01_RS09085 (J8N01_08980) nucA/comI 1379812..1380186 (-) 375 WP_223269005.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  J8N01_RS09090 (J8N01_08985) - 1380479..1380739 (+) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  J8N01_RS09095 (J8N01_08990) - 1380895..1382844 (+) 1950 WP_041816612.1 FUSC family protein -
  J8N01_RS09100 (J8N01_08995) - 1382880..1383698 (-) 819 WP_013058559.1 squalene/phytoene synthase family protein -
  J8N01_RS09105 (J8N01_09000) - 1384024..1384695 (+) 672 WP_013058558.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13661.90 Da        Isoelectric Point: 4.2601

>NTDB_id=558909 J8N01_RS09085 WP_223269005.1 1379812..1380186(-) (nucA/comI) [Priestia megaterium strain HT517]
MGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=558909 J8N01_RS09085 WP_223269005.1 1379812..1380186(-) (nucA/comI) [Priestia megaterium strain HT517]
ATGGGACATGCAGCTTTTTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAACTATGATGCGACGCTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGGGAAGAATCGCTGAAAGGATATCCGACAAAAACAGGCTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGTGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.227

95.968

0.645