Detailed information NTG0558429

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KCV10_RS03065
• Genome accession: NZ_CP073243
• Coordinates: 692942..694060 (+)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain S63-3
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 687942..699060

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KCV10_RS03050	-	690109..690951 (-)	843	WP_019456821.1	undecaprenyl-diphosphate phosphatase	-
KCV10_RS03055	-	691058..691750 (-)	693	WP_212511841.1	YggS family pyridoxal phosphate-dependent enzyme	-
KCV10_RS03060	pilT	691877..692914 (+)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
KCV10_RS03065	pilU	692942..694060 (+)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KCV10_RS03070	fur	694131..694568 (-)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
KCV10_RS03075	-	694680..695078 (+)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
KCV10_RS03080	-	695135..695458 (-)	324	WP_019456825.1	RnfH family protein	-
KCV10_RS03085	-	695458..696525 (-)	1068	WP_212511842.1	hypothetical protein	-
KCV10_RS03090	-	696678..697133 (-)	456	WP_004703295.1	bacteriohemerythrin	-
KCV10_RS03095	-	697384..698232 (-)	849	WP_019456827.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=558429 KCV10_RS03065 WP_004703285.1 692942..694060(+) (pilU) [Acinetobacter seifertii strain S63-3]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=558429 KCV10_RS03065 WP_004703285.1 692942..694060(+) (pilU) [Acinetobacter seifertii strain S63-3]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCACCATC
TATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTGATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTTGCGGATACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTTCTACGTCGAATAGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCCTCTGTGCTTAAAGATTTATCAATGACCAAACGCGGCATTATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATCATTACTATTGAA
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTCGGTATTGATACTGACTCGTTCGA
AATTGCCTTGAAAAATACTTTACGACAAGCACCCGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTCAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACCTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTGACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401