Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L1P13_RS20720 Genome accession   NZ_CP091024
Coordinates   4187705..4188145 (+) Length   146 a.a.
NCBI ID   WP_000360894.1    Uniprot ID   -
Organism   Escherichia coli strain STEC379     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4182705..4193145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1P13_RS20700 aroP 4183703..4185076 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  L1P13_RS20705 ampE 4185119..4185973 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  L1P13_RS20710 ampD 4185970..4186521 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L1P13_RS20715 nadC 4186609..4187502 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L1P13_RS20720 pilA 4187705..4188145 (+) 441 WP_000360894.1 prepilin peptidase-dependent pilin Machinery gene
  L1P13_RS20725 pilB 4188155..4189540 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  L1P13_RS20730 hofC 4189530..4190732 (+) 1203 WP_000157234.1 protein transport protein HofC -
  L1P13_RS20735 guaC 4190767..4191810 (-) 1044 WP_001217338.1 GMP reductase -
  L1P13_RS20740 - 4191966..4192010 (-) 45 WP_120795372.1 protein YacM -
  L1P13_RS20745 coaE 4192035..4192655 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15611.72 Da        Isoelectric Point: 4.5538

>NTDB_id=557652 L1P13_RS20720 WP_000360894.1 4187705..4188145(+) (pilA) [Escherichia coli strain STEC379]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=557652 L1P13_RS20720 WP_000360894.1 4187705..4188145(+) (pilA) [Escherichia coli strain STEC379]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377