Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KBU60_RS02930 Genome accession   NZ_CP073068
Coordinates   602370..602834 (-) Length   154 a.a.
NCBI ID   WP_025524817.1    Uniprot ID   A0A7Z2MTQ1
Organism   Vibrio parahaemolyticus strain FB-11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 597370..607834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBU60_RS02910 (KBU60_02910) coaE 597882..598496 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  KBU60_RS02915 (KBU60_02915) pilD 598497..599366 (-) 870 WP_025552578.1 A24 family peptidase Machinery gene
  KBU60_RS02920 (KBU60_02920) pilC 599431..600654 (-) 1224 WP_021453293.1 type II secretion system F family protein Machinery gene
  KBU60_RS02925 (KBU60_02925) pilB 600678..602363 (-) 1686 WP_140287838.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KBU60_RS02930 (KBU60_02930) pilA 602370..602834 (-) 465 WP_025524817.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  KBU60_RS02935 (KBU60_02935) nadC 603098..603985 (-) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KBU60_RS02940 (KBU60_02940) ampD 604078..604629 (+) 552 WP_005479703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KBU60_RS02945 (KBU60_02945) pdhR 605035..605802 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16144.59 Da        Isoelectric Point: 8.1135

>NTDB_id=557652 KBU60_RS02930 WP_025524817.1 602370..602834(-) (pilA) [Vibrio parahaemolyticus strain FB-11]
MKHSKQKKQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKKATLAEFPKVAAAMKLAVELCAHENASDATTFKSNCLSGS
NGVPAKITNLNDISIEAVAGSSGVNVIAQAVVVKGPIAAKEQYIMASNYTGSGIEWTSSCKDAGGKAQTDYCPE

Nucleotide


Download         Length: 465 bp        

>NTDB_id=557652 KBU60_RS02930 WP_025524817.1 602370..602834(-) (pilA) [Vibrio parahaemolyticus strain FB-11]
ATGAAACACAGTAAACAGAAAAAACAACAAGGTTTTACCTTGATTGAATTGATGATTGTAGTGGCTATTATCGGTATTTT
GGCTGCATTTGCCGTACCGGCATATCAAAACTATACAAAAAAAGCGACATTAGCGGAATTCCCCAAAGTAGCAGCAGCTA
TGAAATTAGCAGTTGAGTTGTGCGCACACGAAAATGCTTCGGATGCAACGACTTTTAAATCAAACTGTCTTTCCGGTAGT
AATGGGGTGCCTGCAAAGATTACTAACCTAAATGATATATCAATTGAAGCTGTCGCAGGGTCATCAGGTGTAAATGTTAT
TGCACAAGCAGTTGTAGTAAAAGGACCTATCGCAGCTAAAGAACAATACATAATGGCATCAAACTACACAGGAAGTGGCA
TTGAATGGACTAGCTCTTGTAAAGATGCAGGGGGTAAGGCTCAAACTGATTACTGCCCTGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2MTQ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

58.71

100

0.591
  pilA2 Legionella pneumophila strain ERS1305867

39.865

96.104

0.383
  pilA Haemophilus influenzae 86-028NP

41.429

90.909

0.377