Detailed information NTG0557518

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J7D36_RS13840
• Genome accession: NZ_CP073060
• Coordinates: 2933794..2934912 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain DETAB-P39
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2928794..2939912

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J7D36_RS13830 (J7D36_13830)	-	2931910..2932602 (-)	693	WP_021510937.1	YggS family pyridoxal phosphate-dependent enzyme	-
J7D36_RS13835 (J7D36_13835)	pilT	2932729..2933766 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
J7D36_RS13840 (J7D36_13840)	pilU	2933794..2934912 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
J7D36_RS13845 (J7D36_13845)	fur	2934982..2935419 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
J7D36_RS13850 (J7D36_13850)	-	2935531..2935929 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
J7D36_RS13855 (J7D36_13855)	-	2935984..2936307 (-)	324	WP_000442585.1	RnfH family protein	-
J7D36_RS13860 (J7D36_13860)	-	2936307..2937374 (-)	1068	WP_000092403.1	hypothetical protein	-
J7D36_RS13865 (J7D36_13865)	-	2937527..2937982 (-)	456	WP_000782976.1	bacteriohemerythrin	-
J7D36_RS13870 (J7D36_13870)	-	2938235..2939083 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=557518 J7D36_RS13840 WP_000347036.1 2933794..2934912(+) (pilU) [Acinetobacter baumannii strain DETAB-P39]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=557518 J7D36_RS13840 WP_000347036.1 2933794..2934912(+) (pilU) [Acinetobacter baumannii strain DETAB-P39]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TTATGAGCGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAATTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392