Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   KBW66_RS04970 Genome accession   NZ_CP072980
Coordinates   1050892..1052016 (+) Length   374 a.a.
NCBI ID   WP_000228517.1    Uniprot ID   E2QFE2
Organism   Escherichia coli strain 2547     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1045892..1057016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBW66_RS04945 (KBW66_04945) mscL 1046009..1046422 (-) 414 WP_000022450.1 large-conductance mechanosensitive channel protein MscL -
  KBW66_RS04950 (KBW66_04950) trkA 1046552..1047928 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  KBW66_RS04955 (KBW66_04955) rsmB 1047950..1049239 (-) 1290 WP_000744765.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  KBW66_RS04960 (KBW66_04960) fmt 1049291..1050238 (-) 948 WP_000004421.1 methionyl-tRNA formyltransferase -
  KBW66_RS04965 (KBW66_04965) def 1050253..1050762 (-) 510 WP_000114984.1 peptide deformylase -
  KBW66_RS04970 (KBW66_04970) dprA 1050892..1052016 (+) 1125 WP_000228517.1 DNA-protecting protein DprA Machinery gene
  KBW66_RS04975 (KBW66_04975) smg 1051988..1052461 (+) 474 WP_000460673.1 DUF494 family protein Smg -
  KBW66_RS04980 (KBW66_04980) yrdD 1052490..1053032 (+) 543 WP_001296461.1 type I DNA topoisomerase -
  KBW66_RS04985 (KBW66_04985) tsaC 1053037..1053609 (+) 573 WP_001296460.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  KBW66_RS04990 (KBW66_04990) aroE 1053614..1054432 (+) 819 WP_000451255.1 shikimate dehydrogenase -
  KBW66_RS04995 (KBW66_04995) yrdB 1054429..1054686 (+) 258 WP_001070565.1 DUF1488 domain-containing protein -
  KBW66_RS05000 (KBW66_05000) yrdA 1054662..1055216 (-) 555 WP_000005712.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40910.86 Da        Isoelectric Point: 6.0395

>NTDB_id=557109 KBW66_RS04970 WP_000228517.1 1050892..1052016(+) (dprA) [Escherichia coli strain 2547]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=557109 KBW66_RS04970 WP_000228517.1 1050892..1052016(+) (dprA) [Escherichia coli strain 2547]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT
TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAGGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E2QFE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39