NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	J9B93_RS11785	Genome accession	NZ_CP072843
Coordinates	2459306..2460376 (-)	Length	356 a.a.
NCBI ID	WP_003153083.1	Uniprot ID	-
Organism	Bacillus velezensis strain VY03
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2454306..2465376

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J9B93_RS11735 (J9B93_11735)	sipW	2454366..2454950 (-)	585	WP_012117977.1	signal peptidase I SipW	-
J9B93_RS11740 (J9B93_11740)	tapA	2454922..2455593 (-)	672	WP_063174755.1	amyloid fiber anchoring/assembly protein TapA	-
J9B93_RS11745 (J9B93_11745)	-	2455852..2456181 (+)	330	WP_003153097.1	DUF3889 domain-containing protein	-
J9B93_RS11750 (J9B93_11750)	-	2456221..2456400 (-)	180	WP_003153093.1	YqzE family protein	-
J9B93_RS11755 (J9B93_11755)	comGG	2456457..2456834 (-)	378	WP_014305410.1	competence type IV pilus minor pilin ComGG	Machinery gene
J9B93_RS11760 (J9B93_11760)	comGF	2456835..2457230 (-)	396	WP_095318401.1	competence type IV pilus minor pilin ComGF	-
J9B93_RS11765 (J9B93_11765)	comGE	2457244..2457558 (-)	315	WP_041481885.1	competence type IV pilus minor pilin ComGE	Machinery gene
J9B93_RS11770 (J9B93_11770)	comGD	2457542..2457979 (-)	438	WP_095318402.1	competence type IV pilus minor pilin ComGD	Machinery gene
J9B93_RS11775 (J9B93_11775)	comGC	2457969..2458235 (-)	267	WP_042635730.1	competence type IV pilus major pilin ComGC	Machinery gene
J9B93_RS11780 (J9B93_11780)	comGB	2458282..2459319 (-)	1038	WP_095318403.1	competence type IV pilus assembly protein ComGB	Machinery gene
J9B93_RS11785 (J9B93_11785)	comGA	2459306..2460376 (-)	1071	WP_003153083.1	competence type IV pilus ATPase ComGA	Machinery gene
J9B93_RS11790 (J9B93_11790)	-	2460568..2461518 (-)	951	WP_014305415.1	magnesium transporter CorA family protein	-
J9B93_RS11795 (J9B93_11795)	-	2461664..2462965 (+)	1302	WP_014305416.1	hemolysin family protein	-
J9B93_RS11800 (J9B93_11800)	-	2463003..2463833 (-)	831	WP_003153080.1	STAS domain-containing protein	-
J9B93_RS11810 (J9B93_11810)	-	2464039..2464419 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
J9B93_RS11815 (J9B93_11815)	-	2464656..2464901 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40529.85 Da Isoelectric Point: 9.4729

                                >NTDB_id=556112 J9B93_RS11785 WP_003153083.1 2459306..2460376(-) (comGA) [Bacillus velezensis strain VY03]

MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=556112 J9B93_RS11785 WP_003153083.1 2459306..2460376(-) (comGA) [Bacillus velezensis strain VY03]

GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAACGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT

GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG

GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC

CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA

GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATAGCGCTCGGCTATGTCACAACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.022	100	0.82
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0556112