NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	J8615_RS12540	Genome accession	NZ_CP072791
Coordinates	2563112..2564182 (-)	Length	356 a.a.
NCBI ID	WP_003153083.1	Uniprot ID	-
Organism	Bacillus velezensis strain GS-1
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2558112..2569182

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J8615_RS12490 (J8615_12480)	sipW	2558172..2558756 (-)	585	WP_046559873.1	signal peptidase I SipW	-
J8615_RS12495 (J8615_12485)	tapA	2558728..2559399 (-)	672	WP_046559874.1	amyloid fiber anchoring/assembly protein TapA	-
J8615_RS12500 (J8615_12490)	-	2559658..2559987 (+)	330	WP_003153097.1	DUF3889 domain-containing protein	-
J8615_RS12505 (J8615_12495)	-	2560027..2560206 (-)	180	WP_003153093.1	YqzE family protein	-
J8615_RS12510 (J8615_12500)	comGG	2560263..2560640 (-)	378	WP_046559875.1	competence type IV pilus minor pilin ComGG	Machinery gene
J8615_RS12515 (J8615_12505)	comGF	2560641..2561036 (-)	396	WP_046559876.1	competence type IV pilus minor pilin ComGF	-
J8615_RS12520 (J8615_12510)	comGE	2561050..2561364 (-)	315	WP_015388003.1	competence type IV pilus minor pilin ComGE	Machinery gene
J8615_RS12525 (J8615_12515)	comGD	2561348..2561785 (-)	438	WP_044053464.1	competence type IV pilus minor pilin ComGD	Machinery gene
J8615_RS12530 (J8615_12520)	comGC	2561775..2562083 (-)	309	WP_219984632.1	competence type IV pilus major pilin ComGC	Machinery gene
J8615_RS12535 (J8615_12525)	comGB	2562088..2563125 (-)	1038	WP_014305414.1	competence type IV pilus assembly protein ComGB	Machinery gene
J8615_RS12540 (J8615_12530)	comGA	2563112..2564182 (-)	1071	WP_003153083.1	competence type IV pilus ATPase ComGA	Machinery gene
J8615_RS12545 (J8615_12535)	-	2564374..2565324 (-)	951	WP_003153082.1	magnesium transporter CorA family protein	-
J8615_RS12550 (J8615_12540)	-	2565470..2566771 (+)	1302	WP_014305416.1	hemolysin family protein	-
J8615_RS12555 (J8615_12545)	-	2566810..2567640 (-)	831	WP_003153080.1	STAS domain-containing protein	-
J8615_RS12565 (J8615_12555)	-	2567846..2568226 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
J8615_RS12570 (J8615_12560)	-	2568462..2568707 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40529.85 Da Isoelectric Point: 9.4729

                                >NTDB_id=555843 J8615_RS12540 WP_003153083.1 2563112..2564182(-) (comGA) [Bacillus velezensis strain GS-1]

MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=555843 J8615_RS12540 WP_003153083.1 2563112..2564182(-) (comGA) [Bacillus velezensis strain GS-1]

GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGTTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT

GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG

GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC

CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA

GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.022	100	0.82
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0555843