Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LZ756_RS07460 Genome accession   NZ_CP090547
Coordinates   1561628..1562686 (+) Length   352 a.a.
NCBI ID   WP_004089911.1    Uniprot ID   B2I8B6
Organism   Xylella fastidiosa subsp. sandyi strain OC8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1556628..1567686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZ756_RS07450 (LZ756_07450) - 1557675..1558640 (+) 966 WP_020852829.1 aldo/keto reductase -
  LZ756_RS07455 (LZ756_07455) - 1558945..1561374 (-) 2430 WP_024749302.1 penicillin-binding protein 1A -
  LZ756_RS07460 (LZ756_07460) pilM 1561628..1562686 (+) 1059 WP_004089911.1 type IV pilus biogenesis protein PilM Machinery gene
  LZ756_RS07465 (LZ756_07465) - 1562686..1563264 (+) 579 Protein_1455 PilN domain-containing protein -
  LZ756_RS07470 (LZ756_07470) - 1563348..1564016 (+) 669 WP_004089909.1 type 4a pilus biogenesis protein PilO -
  LZ756_RS07475 (LZ756_07475) - 1564013..1564543 (+) 531 WP_011098214.1 pilus assembly protein PilP -
  LZ756_RS07480 (LZ756_07480) comQ/pilQ 1564543..1566456 (+) 1914 WP_038274677.1 type IV pilus secretin PilQ Machinery gene

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37539.19 Da        Isoelectric Point: 4.6541

>NTDB_id=554843 LZ756_RS07460 WP_004089911.1 1561628..1562686(+) (pilM) [Xylella fastidiosa subsp. sandyi strain OC8]
MGLFSKKQSVLVGVDISSTAVKLLQLSRSGNRFKVEHYAVEPLPLNAVAEKGIVEVEQVGEAIRRAVSRSGTKAKFAAAA
VAGSAVITKLIPMPAGLDEQDLEAQIEIEATNYIPYPIEEVSLDFEVLGPVPNNTEMVQVLLAASRSENVELRQSALELG
GLTAKVIDVEALAVENAFSLIAQELSVGSNALVALIDIGATMSTLNVLHSGRSLYAREQLFGGKQLTDEVMHRYGMTYEE
AGQAKRQGGLPQSYEVEVLGPFKDSVIQQISRLLQFFYAGSEYNRVDCIVLAGGCAVIAGLPAMVEERLGVVTVVANPLA
QMTLGAKVQAHTLAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=554843 LZ756_RS07460 WP_004089911.1 1561628..1562686(+) (pilM) [Xylella fastidiosa subsp. sandyi strain OC8]
GTGGGGCTTTTTTCAAAAAAACAGTCGGTATTGGTTGGTGTCGACATTAGTTCAACTGCAGTGAAGCTCTTACAGCTTTC
CCGCAGTGGTAATCGCTTCAAGGTGGAACACTATGCTGTGGAGCCGCTACCTCTGAATGCGGTCGCCGAGAAGGGTATCG
TTGAAGTCGAGCAGGTTGGTGAAGCAATCCGTCGTGCGGTAAGTCGTTCTGGAACTAAGGCCAAGTTTGCCGCTGCCGCG
GTTGCCGGTTCGGCAGTGATCACCAAGTTGATACCGATGCCTGCTGGTCTGGACGAGCAGGATTTGGAAGCTCAGATAGA
AATCGAGGCTACGAATTACATCCCGTACCCTATTGAAGAGGTCAGCCTAGATTTCGAGGTGCTTGGTCCGGTTCCCAACA
ACACGGAGATGGTCCAAGTTTTGCTGGCTGCGTCTCGTTCGGAGAATGTCGAATTGCGCCAATCTGCGTTGGAGCTGGGT
GGGTTGACTGCCAAGGTTATTGACGTGGAAGCTTTAGCGGTTGAGAACGCTTTCTCTCTTATTGCCCAAGAACTGTCGGT
TGGGAGTAATGCATTGGTTGCATTGATCGACATCGGTGCAACGATGTCGACGCTTAATGTGCTGCATAGTGGTCGCAGTT
TGTACGCGCGCGAGCAGCTATTTGGTGGTAAGCAGCTTACTGACGAAGTAATGCATCGCTATGGGATGACTTACGAGGAG
GCTGGTCAGGCCAAGCGTCAAGGAGGTTTGCCGCAAAGCTATGAGGTTGAAGTGCTGGGACCATTTAAGGACTCTGTGAT
CCAGCAGATCAGCCGTTTATTACAGTTTTTCTATGCTGGCAGTGAGTACAACCGAGTTGATTGCATCGTGTTGGCGGGAG
GCTGTGCTGTGATTGCGGGGTTACCGGCTATGGTCGAAGAACGGTTGGGTGTCGTTACCGTTGTGGCTAATCCGCTTGCG
CAGATGACTTTAGGGGCGAAAGTGCAAGCACACACGCTTGCTCAAGATGCACCTGCTTTAATGATTGCTACTGGTCTGGC
TTTGAGGAGTTTTGACTGA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B2I8B6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

48.864

100

0.489
  comM Acinetobacter nosocomialis M2

48.864

100

0.489
  comM/pilM Acinetobacter baylyi ADP1

48.011

100

0.48
  pilM Legionella pneumophila strain ERS1305867

45.609

100

0.457