Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   J5226_RS24820 Genome accession   NZ_CP072597
Coordinates   5854350..5855408 (-) Length   352 a.a.
NCBI ID   WP_215837751.1    Uniprot ID   -
Organism   Lysobacter sp. K5869     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5849350..5860408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5226_RS24800 (J5226_24800) pilQ 5850153..5852099 (-) 1947 WP_215840566.1 type IV pilus secretin PilQ -
  J5226_RS24805 (J5226_24805) - 5852274..5852828 (-) 555 WP_215837748.1 pilus assembly protein PilP -
  J5226_RS24810 (J5226_24810) pilO 5852831..5853532 (-) 702 WP_215837749.1 type 4a pilus biogenesis protein PilO -
  J5226_RS24815 (J5226_24815) - 5853529..5854350 (-) 822 WP_215837750.1 PilN domain-containing protein -
  J5226_RS24820 (J5226_24820) pilM 5854350..5855408 (-) 1059 WP_215837751.1 pilus assembly protein PilM Machinery gene
  J5226_RS24825 (J5226_24825) - 5855997..5858543 (+) 2547 WP_215837752.1 penicillin-binding protein 1A -
  J5226_RS24830 (J5226_24830) - 5858846..5859499 (+) 654 WP_215837753.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37685.04 Da        Isoelectric Point: 4.6860

>NTDB_id=554473 J5226_RS24820 WP_215837751.1 5854350..5855408(-) (pilM) [Lysobacter sp. K5869]
MGLITKSQPALVGVDISSTAVKLLQLSRAGNRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIKRAVARSGTRVKHAAAA
VAGSSVITRVIGMPADLDGDDLESQVELEAANYVPYPIDEVNHDFEVLGPMPGSPDMVQVLLAASRSENVEVRASALELG
GLTPRVMDVEAFAIENAFALLADTLSSPRDGLVALVDSGATMTTLNVLRNGRSLYHREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYQAEVLEPFKEAMVQQVSRLLQFFYAGSEFNRVDQIVLAGGGASIPRIAEMVEEQLGIPTTVANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=554473 J5226_RS24820 WP_215837751.1 5854350..5855408(-) (pilM) [Lysobacter sp. K5869]
GTGGGACTCATCACAAAAAGCCAGCCGGCTCTCGTCGGCGTGGACATCAGTTCGACCGCGGTAAAGTTGCTGCAACTTTC
GCGTGCGGGCAATCGCTACCGCGTTGAGCATTACGCGGTCGAGCCGTTGCCGCCGAACGCGGTGGTGGAAAAGAACATCG
TCGAGGTCGAAGCGGTGGGCGAGGCGATCAAGCGCGCCGTGGCCCGCTCGGGGACCCGCGTCAAGCACGCCGCCGCGGCG
GTCGCCGGTTCCTCGGTGATCACCCGCGTGATCGGCATGCCGGCCGACCTGGACGGGGACGATCTGGAGTCGCAGGTCGA
GCTGGAAGCGGCCAATTACGTGCCGTATCCGATCGATGAGGTCAACCACGATTTCGAGGTGCTCGGCCCGATGCCGGGCA
GCCCGGACATGGTCCAGGTGCTGCTCGCGGCCTCGCGTTCCGAGAACGTCGAGGTGCGCGCCTCGGCGCTCGAGCTCGGC
GGCCTGACGCCCAGGGTCATGGACGTGGAAGCCTTCGCGATCGAGAACGCGTTCGCGCTGCTGGCCGACACGCTGAGCAG
CCCGCGCGACGGTCTGGTCGCCCTGGTCGACTCCGGCGCGACGATGACCACGCTCAACGTGTTGCGCAACGGCCGCAGCC
TCTATCACCGCGAACAGGTGTTCGGCGGCAAGCAGCTGACCGACGAGGTCATGCGCCGCTACGGCCTGAGCTACGAGGAA
GCCGGTCTGGCCAAGCGCCAGGGCGGGCTGCCGGAGAGCTATCAGGCCGAGGTGCTGGAGCCGTTCAAGGAAGCCATGGT
CCAGCAGGTCAGCCGCCTGCTGCAGTTCTTCTATGCCGGCAGCGAGTTCAACCGGGTCGACCAGATCGTTCTGGCCGGCG
GCGGCGCCTCGATTCCGCGCATCGCCGAAATGGTGGAAGAGCAGCTCGGCATTCCGACCACGGTCGCCAACCCGCTCGCC
CACATGACCCTGGGGCCGCGCGTGCAGGCGCACGCGCTGGCGCAGGACGCTCCCGCGCTGATGATCGCCTGCGGCCTGGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.568

100

0.506
  comM Acinetobacter nosocomialis M2

50.284

100

0.503
  comM Acinetobacter baylyi ADP1

50

99.432

0.497
  pilM Legionella pneumophila strain ERS1305867

43.909

100

0.44