Detailed information NTG0554121

Bioinformatically predicted

Overview

• Name: comE
• Type: Machinery gene
• Locus tag: J7649_RS08795
• Genome accession: NZ_CP072549
• Coordinates: 1845124..1845657 (-)
• Length: 177 a.a.
• NCBI ID: WP_219310084.1
• Uniprot ID: -
• Organism: Acinetobacter lwoffii strain H7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1845124..1861076	1845124..1845657	within	0

Gene organization within MGE regions

Location: 1845124..1861076

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J7649_RS08795 (J7649_08795)	comE	1845124..1845657 (-)	534	WP_219310084.1	type IV pilin protein	Machinery gene
J7649_RS08800 (J7649_08800)	-	1845662..1849579 (-)	3918	WP_219307455.1	hypothetical protein	-
J7649_RS08805 (J7649_08805)	-	1849594..1850346 (-)	753	WP_219307457.1	pilus assembly PilX family protein	-
J7649_RS08810 (J7649_08810)	-	1850356..1851360 (-)	1005	WP_219307459.1	PilW family protein	-
J7649_RS08815 (J7649_08815)	pilV	1851360..1851881 (-)	522	WP_219310085.1	type IV pilus modification protein PilV	-
J7649_RS08820 (J7649_08820)	-	1851881..1852441 (-)	561	WP_219307461.1	pilus assembly FimT family protein	-
J7649_RS08825 (J7649_08825)	ispH	1852605..1853555 (-)	951	WP_219307463.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
J7649_RS08830 (J7649_08830)	gmk	1853690..1854313 (+)	624	WP_064094584.1	guanylate kinase	-
J7649_RS08835 (J7649_08835)	rpoZ	1854390..1854671 (+)	282	WP_004281186.1	DNA-directed RNA polymerase subunit omega	-
J7649_RS08840 (J7649_08840)	-	1854885..1856990 (+)	2106	WP_191111828.1	RelA/SpoT family protein	-
J7649_RS08845 (J7649_08845)	-	1857066..1857449 (+)	384	WP_004644973.1	RidA family protein	-
J7649_RS08850 (J7649_08850)	-	1857669..1857863 (+)	195	WP_004644972.1	bacterioferritin-associated ferredoxin	-
J7649_RS08855 (J7649_08855)	bfr	1858100..1858564 (+)	465	WP_005101322.1	heteropolymeric bacterioferritin subunit Bfr	-
J7649_RS08860 (J7649_08860)	-	1858614..1860248 (-)	1635	WP_219307466.1	PglL family O-oligosaccharyltransferase	-
J7649_RS08865 (J7649_08865)	tfpZ	1860336..1861070 (-)	735	WP_219310086.1	TfpX/TfpZ family type IV pilin accessory protein	-

Sequence

Protein

• Length: 177 a.a.
• Molecular weight: 19528.61 Da
• Isoelectric Point: 10.0859

>NTDB_id=554121 J7649_RS08795 WP_219310084.1 1845124..1845657(-) (comE) [Acinetobacter lwoffii strain H7]
MIKLCKKKLKLNQFNQGFTLIELMVVVVIIAILAAIAIPSYQHFVRRGVAAQAQQEMQKIAEQLERHKSRNFSYRGFNAS
YLYPTPSAPKVSSFDASKQLLTLPLESSTIKYTLSLVDSMTGNPLLISSTSTGQGWAIKAVSEDPQNFNFLLTSTGVRCK
QKATITGYASCSAGDNW

Nucleotide

• Length: 534 bp

>NTDB_id=554121 J7649_RS08795 WP_219310084.1 1845124..1845657(-) (comE) [Acinetobacter lwoffii strain H7]
ATGATTAAGCTTTGTAAAAAAAAATTAAAACTGAATCAGTTCAATCAAGGTTTTACCTTGATTGAACTGATGGTAGTAGT
TGTTATCATCGCAATTCTTGCTGCAATCGCCATTCCAAGTTATCAACATTTTGTACGTCGGGGTGTTGCAGCTCAAGCAC
AGCAAGAAATGCAAAAGATAGCTGAACAGTTAGAACGCCATAAATCTCGAAATTTTAGTTATCGTGGATTTAATGCAAGC
TATTTGTACCCAACACCGAGTGCACCTAAAGTATCTTCATTTGATGCTAGTAAACAATTGTTGACACTGCCATTAGAAAG
TTCAACGATCAAATATACATTATCTCTTGTTGATAGCATGACAGGTAACCCACTTTTAATATCTAGCACTTCTACTGGAC
AAGGTTGGGCTATTAAAGCTGTAAGTGAAGATCCTCAAAATTTTAATTTTTTACTTACGAGCACAGGGGTGCGTTGTAAG
CAAAAAGCTACCATAACGGGTTATGCGTCTTGTTCTGCGGGGGATAATTGGTGA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (15-37)

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (39-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Acinetobacter baylyi ADP1	58.824	96.045	0.565
pilY2	Acinetobacter baumannii D1279779	46.622	83.616	0.39