Detailed information NTG0055394

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSEST_RS01415
• Genome accession: NC_019936
• Coordinates: 301434..302579 (+)
• Length: 381 a.a.
• NCBI ID: WP_015275295.1
• Uniprot ID: A0A2N8SGR5
• Organism: Stutzerimonas stutzeri RCH2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 296434..307579

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSEST_RS01395 (Psest_0282)	-	298149..298739 (-)	591	WP_015275292.1	YggT family protein	-
PSEST_RS01400 (Psest_0283)	proC	298749..299573 (-)	825	WP_015275293.1	pyrroline-5-carboxylate reductase	-
PSEST_RS01405 (Psest_0284)	-	299584..300273 (-)	690	WP_015275294.1	YggS family pyridoxal phosphate-dependent enzyme	-
PSEST_RS01410 (Psest_0285)	pilT	300340..301374 (+)	1035	WP_003303153.1	type IV pilus twitching motility protein PilT	Machinery gene
PSEST_RS01415 (Psest_0286)	pilU	301434..302579 (+)	1146	WP_015275295.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSEST_RS01420 (Psest_0287)	-	302576..303412 (-)	837	WP_015275296.1	TIGR03915 family putative DNA repair protein	-
PSEST_RS01425 (Psest_0288)	-	303412..304623 (-)	1212	WP_015275297.1	putative DNA modification/repair radical SAM protein	-
PSEST_RS01430 (Psest_0289)	-	304757..305152 (+)	396	WP_003282737.1	NINE protein	-
PSEST_RS01435 (Psest_0290)	-	305220..306494 (-)	1275	WP_015275298.1	dihydroorotase	-
PSEST_RS01440 (Psest_0291)	-	306494..307510 (-)	1017	WP_015275299.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42430.68 Da
• Isoelectric Point: 6.7256

>NTDB_id=55394 PSEST_RS01415 WP_015275295.1 301434..302579(+) (pilU) [Stutzerimonas stutzeri RCH2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDDDPGRSFR

Nucleotide

• Length: 1146 bp

>NTDB_id=55394 PSEST_RS01415 WP_015275295.1 301434..302579(+) (pilU) [Stutzerimonas stutzeri RCH2]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCAGGCGTACCGCCTTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGGCGATGTCGCCGGAAATGACCCGCGAGACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGTATCGGCCGT
TTCCGGGTCAGCGCCTTCTACCAGCGCAACCTCGCCGGCATGGTGCTGCGGCGTATCGAAACCAACATTCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTCAAGAAGCTGTCGATGACCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGCAAGTCGACCTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAATTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCATCAGAGCTGCATCGTCACCCAGCGCGAGGTCGGTATCGACACCAGCTCGTTCGAGGT
GGCCTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGCACCCGTGAAACCATGGACTACG
CCGTAGCGTTCGCCGAAACCGGTCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGCCTGACCGGCACAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACGCCTGACGGCAAGGGGCGCCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGATCTGA
TCCGCAAGGGCGAGGTGCATGAGCTCAAGTCGCTGATGAAGCGTTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGCGAGATCACCTACGAAGATGCGTTGCTGCACGCCGATTCGGCCAACGACCTGCGGCT
GCTGATCAAGCTGGGATCGGAAACCGATGGCGAACATCTGACCAGCGTGTCGCAAGGCCTGAGCCTCGAGGTCAGTGACG
ACGATCCGGGCCGCAGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: A0A2N8SGR5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.013	100	0.95
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388