Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   LXN03_RS26850 Genome accession   NZ_CP090348
Coordinates   5787010..5787633 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PA8329     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5782010..5792633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXN03_RS26830 (LXN03_26830) aroB 5782605..5783711 (-) 1107 WP_004352494.1 3-dehydroquinate synthase -
  LXN03_RS26835 (LXN03_26835) aroK 5783761..5784279 (-) 519 WP_003095833.1 shikimate kinase AroK -
  LXN03_RS26840 (LXN03_26840) pilQ 5784291..5786435 (-) 2145 WP_003103267.1 type 4a pilus secretin PilQ Machinery gene
  LXN03_RS26845 (LXN03_26845) comL/pilP 5786489..5787013 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  LXN03_RS26850 (LXN03_26850) pilO 5787010..5787633 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  LXN03_RS26855 (LXN03_26855) pilN 5787630..5788226 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  LXN03_RS26860 (LXN03_26860) pilM 5788226..5789290 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  LXN03_RS26865 (LXN03_26865) - 5789475..5791946 (+) 2472 WP_004352493.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=553689 LXN03_RS26850 WP_003103268.1 5787010..5787633(-) (pilO) [Pseudomonas aeruginosa strain PA8329]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=553689 LXN03_RS26850 WP_003103268.1 5787010..5787633(-) (pilO) [Pseudomonas aeruginosa strain PA8329]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGTGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAATGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401