Detailed information NTG0552360

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J6B58_RS16295
• Genome accession: NZ_CP072398
• Coordinates: 3409672..3410790 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain KSK2
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3404672..3415790

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J6B58_RS16285 (J6B58_16285)	-	3407788..3408480 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
J6B58_RS16290 (J6B58_16290)	pilT	3408607..3409644 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
J6B58_RS16295 (J6B58_16295)	pilU	3409672..3410790 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
J6B58_RS16300 (J6B58_16300)	fur	3410860..3411297 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
J6B58_RS16305 (J6B58_16305)	-	3411409..3411807 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
J6B58_RS16310 (J6B58_16310)	-	3411862..3412185 (-)	324	WP_000442590.1	RnfH family protein	-
J6B58_RS16315 (J6B58_16315)	-	3412185..3413252 (-)	1068	WP_004748097.1	hypothetical protein	-
J6B58_RS16320 (J6B58_16320)	-	3413405..3413860 (-)	456	WP_000782976.1	bacteriohemerythrin	-
J6B58_RS16325 (J6B58_16325)	-	3414113..3414961 (-)	849	WP_025469635.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=552360 J6B58_RS16295 WP_000347036.1 3409672..3410790(+) (pilU) [Acinetobacter baumannii strain KSK2]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=552360 J6B58_RS16295 WP_000347036.1 3409672..3410790(+) (pilU) [Acinetobacter baumannii strain KSK2]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAATAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392