Detailed information NTG0551710

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J6I82_RS15105
• Genome accession: NZ_CP072300
• Coordinates: 3159430..3160548 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain KSK20
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3154430..3165548

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J6I82_RS15095 (J6I82_15095)	-	3157546..3158238 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
J6I82_RS15100 (J6I82_15100)	pilT	3158365..3159402 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
J6I82_RS15105 (J6I82_15105)	pilU	3159430..3160548 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
J6I82_RS15110 (J6I82_15110)	fur	3160618..3161055 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
J6I82_RS15115 (J6I82_15115)	-	3161167..3161565 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
J6I82_RS15120 (J6I82_15120)	-	3161620..3161943 (-)	324	WP_000442590.1	RnfH family protein	-
J6I82_RS15125 (J6I82_15125)	-	3161943..3163010 (-)	1068	WP_004748097.1	hypothetical protein	-
J6I82_RS15130 (J6I82_15130)	-	3163163..3163618 (-)	456	WP_000782976.1	bacteriohemerythrin	-
J6I82_RS15135 (J6I82_15135)	-	3163871..3164719 (-)	849	WP_025469635.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=551710 J6I82_RS15105 WP_000347036.1 3159430..3160548(+) (pilU) [Acinetobacter baumannii strain KSK20]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=551710 J6I82_RS15105 WP_000347036.1 3159430..3160548(+) (pilU) [Acinetobacter baumannii strain KSK20]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAATAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392