Detailed information NTG0551428

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J6E47_RS15080
• Genome accession: NZ_CP072270
• Coordinates: 3154861..3155979 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain KSK6
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3149861..3160979

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J6E47_RS15070 (J6E47_15070)	-	3152977..3153669 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
J6E47_RS15075 (J6E47_15075)	pilT	3153796..3154833 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
J6E47_RS15080 (J6E47_15080)	pilU	3154861..3155979 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
J6E47_RS15085 (J6E47_15085)	fur	3156049..3156486 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
J6E47_RS15090 (J6E47_15090)	-	3156598..3156996 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
J6E47_RS15095 (J6E47_15095)	-	3157051..3157374 (-)	324	WP_000442590.1	RnfH family protein	-
J6E47_RS15100 (J6E47_15100)	-	3157374..3158441 (-)	1068	WP_004748097.1	hypothetical protein	-
J6E47_RS15105 (J6E47_15105)	-	3158594..3159049 (-)	456	WP_000782976.1	bacteriohemerythrin	-
J6E47_RS15110 (J6E47_15110)	-	3159302..3160150 (-)	849	WP_025469635.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=551428 J6E47_RS15080 WP_000347036.1 3154861..3155979(+) (pilU) [Acinetobacter baumannii strain KSK6]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=551428 J6E47_RS15080 WP_000347036.1 3154861..3155979(+) (pilU) [Acinetobacter baumannii strain KSK6]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAATAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392