Detailed information    

insolico Bioinformatically predicted

Overview


Name   comYF   Type   Machinery gene
Locus tag   J5M87_RS00635 Genome accession   NZ_CP072115
Coordinates   99096..99533 (+) Length   145 a.a.
NCBI ID   WP_154607860.1    Uniprot ID   A0A6I4RAZ3
Organism   Streptococcus zhangguiae strain zg-86     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 97172..121543 99096..99533 within 0


Gene organization within MGE regions


Location: 97172..121543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5M87_RS00620 (J5M87_00620) comGC/cglC 98145..98459 (+) 315 WP_154607862.1 competence type IV pilus major pilin ComGC Machinery gene
  J5M87_RS00625 (J5M87_00625) comGD/cglD 98485..98847 (+) 363 WP_244315564.1 competence type IV pilus minor pilin ComGD Machinery gene
  J5M87_RS00630 (J5M87_00630) comGE 98819..99112 (+) 294 WP_160463141.1 competence type IV pilus minor pilin ComGE -
  J5M87_RS00635 (J5M87_00635) comYF 99096..99533 (+) 438 WP_154607860.1 competence type IV pilus minor pilin ComGF Machinery gene
  J5M87_RS00640 (J5M87_00640) comGG 99544..99969 (+) 426 WP_154607859.1 competence type IV pilus minor pilin ComGG -
  J5M87_RS00645 (J5M87_00645) - 100330..100782 (-) 453 WP_154607858.1 type II toxin-antitoxin system HicB family antitoxin -
  J5M87_RS00650 (J5M87_00650) - 100820..100999 (-) 180 WP_068993248.1 type II toxin-antitoxin system HicA family toxin -
  J5M87_RS00655 (J5M87_00655) - 101183..101590 (-) 408 WP_154607857.1 ArpU family transcriptional regulator -
  J5M87_RS00660 (J5M87_00660) - 102017..102502 (-) 486 WP_154607856.1 hypothetical protein -
  J5M87_RS09770 - 102595..103116 (-) 522 WP_230082343.1 hypothetical protein -
  J5M87_RS00670 (J5M87_00670) - 103129..103377 (-) 249 WP_154607855.1 hypothetical protein -
  J5M87_RS00675 (J5M87_00675) - 103480..104277 (-) 798 WP_154607854.1 DnaD domain-containing protein -
  J5M87_RS00680 (J5M87_00680) - 104431..104712 (-) 282 WP_154607853.1 hypothetical protein -
  J5M87_RS00685 (J5M87_00685) - 104714..105049 (-) 336 WP_154607852.1 DNA-binding protein -
  J5M87_RS00690 (J5M87_00690) - 105059..105253 (-) 195 WP_154607851.1 hypothetical protein -
  J5M87_RS00695 (J5M87_00695) - 105247..105402 (-) 156 WP_154607850.1 hypothetical protein -
  J5M87_RS00700 (J5M87_00700) - 105399..105722 (-) 324 WP_154607849.1 hypothetical protein -
  J5M87_RS00705 (J5M87_00705) - 105942..106571 (-) 630 WP_154607848.1 Rha family transcriptional regulator -
  J5M87_RS09775 - 106589..106717 (-) 129 WP_348271964.1 helix-turn-helix transcriptional regulator -
  J5M87_RS00715 (J5M87_00715) - 106955..107614 (+) 660 WP_154607847.1 helix-turn-helix transcriptional regulator -
  J5M87_RS00720 (J5M87_00720) dinD 107864..108718 (+) 855 WP_154607846.1 DNA damage-inducible protein D -
  J5M87_RS00725 (J5M87_00725) - 108895..110043 (+) 1149 WP_154607845.1 site-specific integrase -
  J5M87_RS00730 (J5M87_00730) comYH 110143..111096 (+) 954 WP_154607844.1 class I SAM-dependent methyltransferase Machinery gene
  J5M87_RS00735 (J5M87_00735) - 111146..112336 (+) 1191 WP_154607843.1 acetate kinase -
  J5M87_RS00740 (J5M87_00740) - 112612..113472 (+) 861 WP_154607842.1 pyridoxamine kinase -
  J5M87_RS00745 (J5M87_00745) - 113469..113996 (+) 528 WP_154607841.1 ECF transporter S component -
  J5M87_RS00750 (J5M87_00750) - 114226..114780 (+) 555 WP_154607840.1 folate family ECF transporter S component -
  J5M87_RS00755 (J5M87_00755) - 114841..116097 (+) 1257 WP_154607839.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -
  J5M87_RS00760 (J5M87_00760) pepA 116632..117705 (-) 1074 WP_154607838.1 glutamyl aminopeptidase -
  J5M87_RS00765 (J5M87_00765) - 117757..118041 (+) 285 WP_154607837.1 DUF4651 domain-containing protein -
  J5M87_RS00770 (J5M87_00770) - 118038..118358 (+) 321 WP_154607836.1 thioredoxin family protein -
  J5M87_RS00775 (J5M87_00775) ytpR 118362..118994 (+) 633 WP_154607835.1 YtpR family tRNA-binding protein -
  J5M87_RS00780 (J5M87_00780) ssbB 119059..119454 (+) 396 WP_154607834.1 single-stranded DNA-binding protein Machinery gene
  J5M87_RS00785 (J5M87_00785) groES 119620..119904 (+) 285 WP_154607833.1 co-chaperone GroES -
  J5M87_RS00790 (J5M87_00790) groL 119921..121543 (+) 1623 WP_154607832.1 chaperonin GroEL -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 16967.62 Da        Isoelectric Point: 8.7813

>NTDB_id=550516 J5M87_RS00635 WP_154607860.1 99096..99533(+) (comYF) [Streptococcus zhangguiae strain zg-86]
MWKKSKVSAFTLLECLVALLVLSGSLLVFEGLTKLIHHELIYQETGLEKKWLVFADQLRYEFEGVRLVKVENNRLYVDKL
GQQLSFGKSKSDDFRKTNDKGQGYQPMLYQVASVAIHQERDIVQIDITFDNGWERSFVYRFKEKV

Nucleotide


Download         Length: 438 bp        

>NTDB_id=550516 J5M87_RS00635 WP_154607860.1 99096..99533(+) (comYF) [Streptococcus zhangguiae strain zg-86]
ATGTGGAAAAAGAGTAAAGTGTCTGCCTTTACTCTCTTAGAATGCCTAGTGGCTTTGCTTGTTTTGTCAGGAAGTTTACT
GGTTTTTGAGGGATTGACTAAGCTGATACATCATGAGTTGATTTATCAAGAAACAGGTTTAGAAAAAAAGTGGCTGGTTT
TTGCAGATCAATTGCGCTATGAGTTTGAGGGAGTTCGTTTGGTTAAGGTGGAAAACAATCGCCTGTATGTTGATAAATTA
GGGCAGCAACTATCCTTTGGCAAATCAAAATCAGATGATTTTCGAAAAACCAATGATAAAGGCCAAGGCTATCAGCCGAT
GCTATATCAGGTTGCCTCTGTCGCTATTCACCAAGAACGAGACATAGTTCAGATTGATATTACTTTTGACAATGGTTGGG
AGAGAAGTTTTGTTTATCGTTTTAAAGAAAAAGTGTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6I4RAZ3

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

52.482

97.241

0.51

  comYF Streptococcus mutans UA159

51.773

97.241

0.503

  comGF/cglF Streptococcus pneumoniae R6

49.65

98.621

0.49

  comGF/cglF Streptococcus pneumoniae TIGR4

49.65

98.621

0.49

  comGF/cglF Streptococcus pneumoniae Rx1

49.65

98.621

0.49

  comGF/cglF Streptococcus pneumoniae D39

49.65

98.621

0.49

  comGF Lactococcus lactis subsp. cremoris KW2

50

96.552

0.483

  comGF/cglF Streptococcus mitis SK321

47.101

95.172

0.448

  comGF/cglF Streptococcus mitis NCTC 12261

47.101

95.172

0.448