Detailed information NTG0054909

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: BEST7003_RS01645
• Genome accession: NZ_AP012496
• Coordinates: 304427..305548 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis BEST7003
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 299427..310548

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BEST7003_RS01625 (BEST7003_0277)	ycdA	299435..300499 (-)	1065	WP_003234750.1	DUF4352 domain-containing protein	-
BEST7003_RS21680	-	300522..300653 (-)	132	WP_003246334.1	hypothetical protein	-
BEST7003_RS01630 (BEST7003_0278)	ycdB	300827..302245 (+)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
BEST7003_RS01635 (BEST7003_0279)	ycdC	302432..303793 (+)	1362	WP_020861261.1	YcdB/YcdC domain-containing protein	-
BEST7003_RS01640 (BEST7003_0280)	cwlK	303801..304304 (-)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
BEST7003_RS01645 (BEST7003_0281)	rapF	304427..305548 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
BEST7003_RS01650 (BEST7003_0282)	ycdF	305655..306431 (+)	777	WP_003246415.1	SDR family oxidoreductase	-
BEST7003_RS01655 (BEST7003_0283)	ycdG	306456..308141 (+)	1686	WP_003234736.1	alpha-glucosidase	-
BEST7003_RS01660 (BEST7003_0284)	znuA	308329..309288 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
BEST7003_RS01665 (BEST7003_0285)	znuC	309344..310039 (+)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=54909 BEST7003_RS01645 WP_003246478.1 304427..305548(+) (rapF) [Bacillus subtilis BEST7003]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=54909 BEST7003_RS01645 WP_003246478.1 304427..305548(+) (rapF) [Bacillus subtilis BEST7003]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405