Detailed information NTG0548904

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J4H94_RS19210
• Genome accession: NZ_CP071899
• Coordinates: 4096634..4097779 (-)
• Length: 381 a.a.
• NCBI ID: WP_026006585.1
• Uniprot ID: A0AA47I1L4
• Organism: Stutzerimonas frequens strain TPA3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4091634..4102779

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J4H94_RS19185 (J4H94_19185)	-	4091713..4092729 (+)	1017	WP_017245430.1	aspartate carbamoyltransferase catalytic subunit	-
J4H94_RS19190 (J4H94_19190)	-	4092729..4094003 (+)	1275	WP_102838899.1	dihydroorotase	-
J4H94_RS19195 (J4H94_19195)	-	4094061..4094456 (-)	396	WP_017245432.1	TM2 domain-containing protein	-
J4H94_RS19200 (J4H94_19200)	-	4094589..4095803 (+)	1215	WP_063540689.1	putative DNA modification/repair radical SAM protein	-
J4H94_RS19205 (J4H94_19205)	-	4095803..4096627 (+)	825	WP_208216950.1	TIGR03915 family putative DNA repair protein	-
J4H94_RS19210 (J4H94_19210)	pilU	4096634..4097779 (-)	1146	WP_026006585.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
J4H94_RS19215 (J4H94_19215)	pilT	4097839..4098873 (-)	1035	WP_017245436.1	type IV pilus twitching motility protein PilT	Machinery gene
J4H94_RS19220 (J4H94_19220)	-	4098940..4099629 (+)	690	WP_200597336.1	YggS family pyridoxal phosphate-dependent enzyme	-
J4H94_RS19225 (J4H94_19225)	proC	4099640..4100464 (+)	825	WP_208216951.1	pyrroline-5-carboxylate reductase	-
J4H94_RS19230 (J4H94_19230)	-	4100474..4101064 (+)	591	WP_014598006.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42540.84 Da
• Isoelectric Point: 6.8856

>NTDB_id=548904 J4H94_RS19210 WP_026006585.1 4096634..4097779(-) (pilU) [Stutzerimonas frequens strain TPA3]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTTVSQGLSLEVSDDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=548904 J4H94_RS19210 WP_026006585.1 4096634..4097779(-) (pilU) [Stutzerimonas frequens strain TPA3]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATTACGGCGGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACCGCCATGTCACCGGAGATGACCCGCGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGTCGCGAGTTCACCGAAAATCACGAATGCAACTTCGCCATCAGTGCCCGCGGGATCGGTCGC
TTCCGTGTCAGTGCGTTCTATCAGCGCAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATTCCGACCATCGA
AGAGCTGAAGCTGCCTGACGTTCTCAAGAAGCTGTCGATGACCAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CTGGCAAGTCGACCTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAGTTCATCCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAAGTCGGCATCGATACCAGCTCTTTCGAGGT
GGCACTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATTCTCATCGGCGAGATTCGTACCCGCGAGACGATGGACTATG
CCGTAGCCTTCGCCGAGACCGGGCACCTGTGTCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGATCGGCACAACCAGGTCTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACACCCGACGGCAAGGGTCGTCGCGCGGTGATCGAGGTGTTGATCAACACCCCTCTGGCCGCAGACCTTA
TCCGCAAGGGCGAGGTGCATGAACTCAAGTCGCTGATGAAACGCTCCACCGAGCTGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGCGAGATCACCTACGAAGATGCGCTGTTGCACGCCGACTCGGCCAACGACCTGCGGCT
GTTGATCAAGCTGGGTTCGGAAACCGACGGCGAGCACCTGACCACCGTTTCGCAGGGGTTGAGTCTGGAGGTCAGCGACG
ACGATCCGGGTCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.751	100	0.948
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388