Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GEI7407_RS02020 Genome accession   NC_019703
Coordinates   463915..465912 (+) Length   665 a.a.
NCBI ID   WP_015170456.1    Uniprot ID   -
Organism   Geitlerinema sp. PCC 7407     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 458915..470912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GEI7407_RS02010 (GEI7407_0385) dnaK 460469..462421 (-) 1953 WP_015170454.1 molecular chaperone DnaK -
  GEI7407_RS02015 (GEI7407_0386) grpE 462615..463319 (-) 705 WP_015170455.1 nucleotide exchange factor GrpE -
  GEI7407_RS02020 (GEI7407_0387) pilB/pilB1 463915..465912 (+) 1998 WP_015170456.1 GspE/PulE family protein Machinery gene
  GEI7407_RS02025 (GEI7407_0388) pilT 465970..467079 (+) 1110 WP_015170457.1 type IV pilus twitching motility protein PilT Machinery gene
  GEI7407_RS02030 (GEI7407_0389) - 467360..468580 (+) 1221 WP_015170458.1 type II secretion system F family protein -
  GEI7407_RS02035 (GEI7407_0390) - 469020..469655 (+) 636 WP_015170459.1 hypothetical protein -

Sequence

Protein


Download         Length: 665 a.a.        Molecular weight: 74370.26 Da        Isoelectric Point: 4.9946

>NTDB_id=54846 GEI7407_RS02020 WP_015170456.1 463915..465912(+) (pilB/pilB1) [Geitlerinema sp. PCC 7407]
MTNSSSQRRALVVQNNFSPFGNKLIQSGFVNADQMQQALIESRKSGRPLTEVLESLTGQQLPPDLLRQYKKQQLFELKIL
YGVESLDPEINQIPTQNVGSLIDTLIPLDICRRHKLVPLSRSEEQPPSVLVAMVDPDNLEAQDDLNRILRPQRLNLQRLV
ITMEDYQRLISGYLDEQVEKQKQLEIQKSVDVSDINLEALDLEEAPDEMEADLNAAVQDAGAAPVIKLVNQILLKALHEG
VSDIHIEPQEEFLRIRFRKDGVLRQAFDPLPKKIIPAVTARFKIIAELDIAERRQPQDGRIRRVYQDRKIDFRVNTLPSR
YGEKVVLRILDNSTTQLGLDKLITDPDTLNTMKDMVKRPFGLILVTGPTGSGKTTTLYSALAERNDPGVNISTAEDPIEY
TLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARLEEMGVET
FMISSSLIGVLAQRLVRRVCQDCKVPYSPEPSELARFGLSASRAGEVTFYRANTLTPEEIQAARSAGTLCPTCNGIGYKG
RCGVYEVMRVTERLQVLMTEGAPTERIKEVAVEEGMKTLLAYSLDLVQQGYTTLDEVERVTFTDTGLEAELKAKRKSALT
CRVCSAELKPEWLDCPYCTTPRFID

Nucleotide


Download         Length: 1998 bp        

>NTDB_id=54846 GEI7407_RS02020 WP_015170456.1 463915..465912(+) (pilB/pilB1) [Geitlerinema sp. PCC 7407]
ATGACTAACTCCTCCTCGCAGCGCCGCGCCCTCGTTGTACAGAACAACTTCTCCCCCTTTGGCAACAAGCTGATCCAGTC
GGGGTTCGTCAATGCCGACCAGATGCAGCAAGCCCTTATAGAAAGTCGTAAATCCGGGCGGCCCCTGACCGAAGTCCTCG
AATCCCTCACCGGCCAGCAACTGCCGCCCGACCTACTTCGCCAATATAAAAAGCAGCAGCTATTCGAACTCAAAATCCTT
TACGGCGTCGAATCCCTCGATCCGGAGATCAACCAGATCCCAACCCAAAACGTTGGCTCCCTGATTGACACCCTGATTCC
CCTCGACATTTGCCGACGCCACAAACTAGTGCCACTGTCCCGCAGCGAAGAGCAGCCCCCCTCGGTCCTCGTTGCCATGG
TGGACCCCGACAACCTCGAAGCCCAAGATGACCTCAACCGCATCCTCCGGCCCCAGCGCCTGAACCTCCAGCGGCTGGTC
ATCACCATGGAAGACTACCAGCGCCTCATCTCTGGCTACCTCGACGAGCAAGTCGAAAAGCAAAAGCAGCTCGAGATTCA
AAAATCCGTTGACGTCTCCGACATCAACCTCGAAGCCCTAGACCTCGAGGAAGCCCCCGACGAGATGGAAGCCGACCTGA
ACGCCGCCGTTCAGGACGCAGGCGCGGCCCCCGTCATCAAACTCGTCAACCAAATCCTGCTCAAAGCCCTCCACGAAGGC
GTCTCAGACATTCACATCGAGCCCCAAGAAGAATTTCTGCGCATTCGCTTCCGCAAAGACGGCGTTCTTCGCCAAGCCTT
CGATCCCCTACCCAAAAAAATCATCCCCGCCGTTACAGCCCGCTTCAAAATCATCGCCGAGCTAGACATCGCCGAGCGGC
GGCAACCCCAAGACGGCCGGATTCGCCGCGTCTACCAAGATCGCAAAATTGACTTCCGAGTCAACACCCTGCCGAGCCGC
TATGGCGAAAAAGTCGTCCTCCGAATCCTAGACAACTCCACCACCCAGCTAGGACTCGATAAACTCATCACCGATCCGGA
CACGCTCAACACCATGAAAGACATGGTTAAGCGGCCGTTCGGCTTGATCCTAGTCACAGGCCCCACCGGATCTGGTAAAA
CCACCACCCTATACTCCGCCCTAGCCGAGCGCAATGACCCCGGTGTCAACATCAGCACCGCAGAAGACCCGATCGAATAC
ACCTTGCCCGGCTTGACCCAGGTGCAGGTCATTCGAGAAAAAGGGATGGACTTTGCATCGATTCTGCGAGCCTTCCTGCG
CCAAGACCCTGACGTGATTCTGGTGGGTGAGACTCGAGACAAAGAAACTGCCAAAACTGCAATCGAGGCGGCCCTCACGG
GACACTTGGTGCTGACCACCCTACACACCAACGACGCCGCCAGTGCGGTAGCCCGACTGGAAGAAATGGGCGTTGAGACG
TTTATGATCTCCAGCTCACTGATCGGGGTGCTGGCTCAGCGTCTGGTGCGGCGGGTATGCCAGGACTGCAAGGTGCCCTA
CAGCCCAGAGCCCTCAGAGCTTGCACGCTTTGGCTTGTCGGCCTCCCGTGCCGGAGAAGTAACGTTCTATCGTGCCAACA
CCCTCACCCCTGAAGAGATTCAGGCGGCGCGATCGGCAGGAACCCTCTGCCCGACCTGTAACGGGATTGGGTACAAGGGA
CGCTGCGGTGTGTATGAGGTGATGCGCGTAACAGAGCGGCTCCAGGTTCTGATGACTGAGGGCGCGCCCACAGAGCGCAT
CAAAGAAGTTGCCGTTGAAGAAGGGATGAAGACCCTCTTGGCCTACAGCTTGGATTTGGTGCAGCAGGGATACACAACGC
TGGACGAAGTGGAGCGGGTGACCTTTACAGATACAGGCTTGGAGGCTGAACTGAAGGCCAAGCGCAAGAGCGCGCTGACC
TGTCGAGTTTGTAGTGCGGAGCTCAAGCCTGAATGGCTAGATTGTCCCTATTGCACGACGCCCCGTTTTATTGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(70-178)

Type II/IV secretion system protein (T2SSE)

(231-498)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.616

99.549

0.663
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.453

92.932

0.376
  pilF Thermus thermophilus HB27

39.739

92.331

0.367

Multiple sequence alignment