Detailed information NTG0547751

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J2887_RS10850
• Genome accession: NZ_CP071785
• Coordinates: 2365646..2366824 (+)
• Length: 392 a.a.
• NCBI ID: WP_227171159.1
• Uniprot ID: -
• Organism: Marinobacter sp. CA1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2360646..2371824

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J2887_RS10830 (J2887_10795)	leuB	2361264..2362337 (-)	1074	WP_227171156.1	3-isopropylmalate dehydrogenase	-
J2887_RS10835 (J2887_10800)	leuD	2362401..2363048 (-)	648	WP_227171157.1	3-isopropylmalate dehydratase small subunit	-
J2887_RS10840 (J2887_10805)	leuC	2363069..2364487 (-)	1419	WP_227171158.1	3-isopropylmalate dehydratase large subunit	-
J2887_RS10845 (J2887_10810)	-	2364711..2365592 (+)	882	WP_227176100.1	LysR family transcriptional regulator	-
J2887_RS10850 (J2887_10815)	pilU	2365646..2366824 (+)	1179	WP_227171159.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
J2887_RS10855 (J2887_10820)	-	2366825..2367979 (-)	1155	WP_227176101.1	MFS transporter	-
J2887_RS10860 (J2887_10825)	aroC	2367985..2369085 (-)	1101	WP_227171160.1	chorismate synthase	-
J2887_RS10865 (J2887_10830)	prmB	2369143..2370051 (-)	909	WP_227171161.1	50S ribosomal protein L3 N(5)-glutamine methyltransferase	-
J2887_RS10870 (J2887_10835)	-	2370094..2371221 (-)	1128	WP_227171162.1	patatin-like phospholipase family protein	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 43492.84 Da
• Isoelectric Point: 5.7015

>NTDB_id=547751 J2887_RS10850 WP_227171159.1 2365646..2366824(+) (pilU) [Marinobacter sp. CA1]
MGFDDYLKILAVKDGSDLYLSTGAPPCAKFQGTLKPIDRTPLKPGEIKEIAYRIMDQEQIEEFEQELEMNLAYSIPQVGR
FRINIFRQRNELSIVARNIVTEIPNADDLGLPPILKDVVMSKRGLVLFVGATGSGKSTSLAALIDHRNSNSRGHIITIED
PVEYIHRHKKCIVNQREVGVDTRSFHQALKNTLRQAPDVILIGEIRDRATMEHALAFAETGHLCISTLHANNANQALDRI
INFFPEERRPQLLMDLAMNIRAFVSQRLIPTLDGKRCAAIEVLLGTPTISELILRGEIDGIKEIMEKSSNLGMQTFDLAL
FNLWKEGRISQEEALKNADSANNLRLKIKLDGGQPDSPPTVTAGPRPAPAPGKPDGGLSLKLEEPDEDPDQS

Nucleotide

• Length: 1179 bp

>NTDB_id=547751 J2887_RS10850 WP_227171159.1 2365646..2366824(+) (pilU) [Marinobacter sp. CA1]
ATGGGCTTCGACGACTACCTCAAGATCCTTGCCGTCAAAGACGGCTCTGACCTGTACCTCAGCACAGGCGCGCCGCCCTG
CGCCAAGTTCCAGGGCACCCTGAAGCCGATCGACCGAACGCCCCTGAAGCCCGGCGAGATCAAGGAGATCGCCTACCGGA
TCATGGATCAGGAGCAGATCGAGGAATTCGAGCAGGAACTGGAGATGAACCTGGCTTACAGCATTCCCCAGGTAGGCCGG
TTCCGTATCAACATCTTTCGACAGCGCAATGAGCTGTCCATTGTGGCCCGCAATATCGTGACCGAGATTCCCAATGCGGA
TGACCTTGGGCTTCCGCCCATCCTGAAAGATGTGGTGATGTCCAAGCGCGGCCTGGTGCTGTTCGTTGGCGCGACCGGCT
CCGGTAAATCCACTTCCCTGGCGGCACTGATCGATCACCGAAATTCCAACTCCCGGGGTCACATCATCACCATTGAAGAC
CCGGTTGAGTACATTCACCGTCACAAGAAGTGCATCGTCAATCAACGGGAAGTTGGCGTTGATACCCGGAGCTTTCATCA
GGCGCTGAAGAATACCCTGCGACAGGCGCCAGACGTTATCCTGATCGGCGAAATCCGCGACCGGGCAACCATGGAGCACG
CCCTGGCATTCGCCGAAACCGGCCACCTGTGCATCTCCACGCTGCACGCCAACAACGCCAACCAGGCCCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGGCGTCCTCAGTTGCTGATGGACCTGGCCATGAACATTCGCGCCTTTGTGTCACAACG
CCTGATCCCAACCCTGGACGGCAAGCGCTGCGCCGCTATCGAGGTGCTGCTGGGCACGCCCACGATCAGCGAGCTGATCC
TACGTGGCGAGATTGATGGCATTAAAGAGATTATGGAAAAGTCGTCGAACCTTGGCATGCAAACCTTCGATCTGGCGCTG
TTCAACCTATGGAAAGAGGGCAGGATCAGCCAGGAGGAGGCGCTGAAAAACGCGGATTCTGCCAACAACCTCCGCCTCAA
AATCAAGCTGGATGGTGGTCAGCCTGACAGTCCACCAACTGTAACTGCCGGCCCCCGACCGGCACCAGCACCAGGCAAAC
CGGATGGCGGCCTGTCGCTCAAACTGGAAGAACCGGATGAGGACCCGGATCAAAGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (53-249)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	53.69	100	0.538
pilU	Acinetobacter baylyi ADP1	55.966	89.796	0.503
pilU	Vibrio cholerae strain A1552	48.87	90.306	0.441
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.71	93.367	0.38