Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NOS7524_RS20985 Genome accession   NC_019684
Coordinates   5164007..5165113 (-) Length   368 a.a.
NCBI ID   WP_015140489.1    Uniprot ID   K9QZ56
Organism   Nostoc sp. PCC 7524     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5159007..5170113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NOS7524_RS20965 (Nos7524_4314) psaK 5159067..5159345 (+) 279 WP_015140485.1 photosystem I reaction center subunit PsaK -
  NOS7524_RS20970 (Nos7524_4315) - 5159863..5162352 (-) 2490 WP_015140486.1 AMIN domain-containing protein -
  NOS7524_RS20975 (Nos7524_4316) - 5162474..5163241 (-) 768 WP_015140487.1 hypothetical protein -
  NOS7524_RS20980 (Nos7524_4317) - 5163238..5164002 (-) 765 WP_015140488.1 PilN domain-containing protein -
  NOS7524_RS20985 (Nos7524_4318) pilM 5164007..5165113 (-) 1107 WP_015140489.1 type IV pilus biogenesis protein PilM Machinery gene
  NOS7524_RS20990 (Nos7524_4319) - 5165259..5166557 (+) 1299 WP_015140490.1 sugar ABC transporter substrate-binding protein -
  NOS7524_RS20995 (Nos7524_4320) - 5166686..5167267 (+) 582 WP_015140491.1 Uma2 family endonuclease -
  NOS7524_RS21000 (Nos7524_4321) - 5167270..5168544 (-) 1275 WP_041555883.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40432.29 Da        Isoelectric Point: 4.4620

>NTDB_id=54746 NOS7524_RS20985 WP_015140489.1 5164007..5165113(-) (pilM) [Nostoc sp. PCC 7524]
MKSFNSLFGKSHKGVGVEIAPERVNVVQLRKQRQGLKLESLTSVAVPEGIVTDGQINDPPAMAQLIQQALAESKIKTSRV
ATGVPGRESIVRIIPVPAELDDKELREMVLNHEAGLYLPYPREEADVDYQKLGYFVDEDGIEKVQVLLVATRKDVTDTYI
STFEQAGLQIDVLEINSFALIRTIRDRHLRELSPQEAAVLVDIEFDSTEIAIVVNGVPQFSRTVPIGTNQMQMALARAMS
LPASRDMEMLYEMTLSVTPGDAEKTGVTETNPGMAGVLRILGELTDELRRSIDFYLNQSENLEVAQILLAGPGGGLQQLD
EFFTQRLSIPTSQIDPIGCLSLQVDEGKYPATQRSGLGIILGLGMREV

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=54746 NOS7524_RS20985 WP_015140489.1 5164007..5165113(-) (pilM) [Nostoc sp. PCC 7524]
GTGAAAAGTTTCAATAGTCTGTTTGGCAAATCTCATAAAGGGGTGGGTGTTGAAATAGCACCAGAACGAGTGAATGTAGT
TCAGCTACGCAAGCAGCGCCAGGGCTTGAAACTCGAATCTTTAACTTCGGTAGCAGTTCCAGAGGGGATTGTCACCGATG
GACAAATCAATGATCCCCCAGCCATGGCGCAACTAATTCAGCAAGCCTTAGCTGAAAGTAAGATCAAGACTTCACGGGTG
GCTACTGGTGTTCCCGGTAGGGAATCGATTGTGCGGATCATACCTGTACCGGCGGAGTTGGATGACAAAGAACTCCGGGA
GATGGTGTTAAACCATGAAGCAGGGTTGTATTTACCCTATCCCCGTGAAGAAGCTGATGTAGATTATCAAAAACTTGGGT
ACTTTGTAGATGAAGACGGCATTGAAAAGGTACAGGTACTGTTAGTTGCCACCCGCAAGGACGTGACAGATACATATATT
AGTACCTTTGAGCAAGCAGGATTGCAAATTGATGTTTTAGAGATTAACAGTTTTGCACTGATTCGGACAATTCGCGATCG
CCATTTGCGAGAATTAAGCCCACAAGAAGCAGCCGTCTTAGTTGATATAGAGTTCGACAGTACAGAAATTGCGATCGTTG
TCAACGGAGTACCCCAATTTTCGCGGACTGTACCCATTGGTACTAACCAAATGCAAATGGCTTTAGCCAGGGCAATGAGT
CTACCTGCATCACGGGATATGGAAATGTTATATGAAATGACACTTTCTGTAACTCCGGGAGACGCTGAAAAAACTGGAGT
CACAGAAACAAATCCTGGGATGGCTGGTGTATTGCGAATCTTGGGAGAACTCACAGATGAACTACGCCGTTCTATAGATT
TTTACTTAAATCAAAGTGAAAACTTAGAAGTAGCACAGATTTTATTAGCTGGCCCCGGTGGTGGATTGCAACAGCTAGAT
GAGTTCTTTACACAAAGACTCAGTATACCCACTAGCCAAATAGATCCCATTGGGTGTTTGTCCTTGCAAGTTGACGAAGG
TAAGTATCCAGCAACACAACGCTCTGGTTTAGGGATCATCCTTGGTTTAGGGATGCGGGAGGTGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-238)

Type IV pilus assembly protein PilM; (PilM_2)

(283-366)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9QZ56

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

65.385

98.913

0.647

Multiple sequence alignment