Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LP473_RS11050 Genome accession   NZ_CP087699
Coordinates   2192435..2193454 (-) Length   339 a.a.
NCBI ID   WP_043991179.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain IBB417     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2187435..2198454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LP473_RS11010 (LP473_11015) - 2188061..2188309 (-) 249 WP_014570815.1 hypothetical protein -
  LP473_RS12750 - 2188323..2188445 (-) 123 WP_023164646.1 hypothetical protein -
  LP473_RS11015 (LP473_11020) - 2188442..2188624 (-) 183 WP_003130605.1 hypothetical protein -
  LP473_RS11020 (LP473_11025) - 2188688..2188897 (-) 210 WP_023349177.1 helix-turn-helix transcriptional regulator -
  LP473_RS11025 (LP473_11030) - 2189089..2189511 (+) 423 WP_023349178.1 helix-turn-helix transcriptional regulator -
  LP473_RS11030 (LP473_11035) - 2189522..2190106 (+) 585 WP_014570821.1 hypothetical protein -
  LP473_RS11035 (LP473_11040) - 2190162..2190701 (+) 540 WP_023189015.1 PH domain-containing protein -
  LP473_RS11040 (LP473_11045) - 2190827..2192284 (+) 1458 WP_031561103.1 recombinase family protein -
  LP473_RS11045 (LP473_11050) - 2192281..2192421 (-) 141 WP_228777719.1 hypothetical protein -
  LP473_RS11050 (LP473_11055) comGB 2192435..2193454 (-) 1020 WP_043991179.1 competence type IV pilus assembly protein ComGB Machinery gene
  LP473_RS11055 (LP473_11060) comGA 2193402..2194341 (-) 940 Protein_2157 competence type IV pilus ATPase ComGA -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 38327.08 Da        Isoelectric Point: 10.3200

>NTDB_id=546929 LP473_RS11050 WP_043991179.1 2192435..2193454(-) (comGB) [Lactococcus lactis subsp. lactis strain IBB417]
MDISHLLKPRGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKKFTLKMKEGLASGQALSELLESLSFSKNV
VTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQLNSGSNFATLLINHLPLV
FFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVELRQIINLMKKQKSRIFSE
VGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKIDRLMQLIQPLVFIFVALMI
ILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=546929 LP473_RS11050 WP_043991179.1 2192435..2193454(-) (comGB) [Lactococcus lactis subsp. lactis strain IBB417]
ATGGATATCTCACACCTACTGAAGCCAAGAGGGAAAAAATTAAGGCTGATTAAACAAGCCAAACTTATCCAACTGATGGG
AAATCTTTTGAATAGTGGATTTAATTTAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAAAATTTA
CATTGAAAATGAAAGAAGGTCTCGCTTCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTG
GTGACACAACTTGCTTTGGTAGAAGTGCATGGTAACTTGTCAGGGACGATGCAATTGGTTGAACTTCATCTAAAAAAACA
GCTTAAAGTTAAAAATAAATTAGTGGAAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGG
GCTTAAAAAATTATTTACTACCACAACTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTA
TTTTTTTCGTTTGGAGCTTTTATATTTTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAA
TTTTACCTTTCTTGTTAAATTACCTCTGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGG
GAAATCTAATTGCTCAAGGTGTTGAATTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAA
GTTGGCAAGAATCTTGATTTAGAATTGAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACC
CGAGCTCTCATTAATGATTGAATATGGAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTT
GGGAACATTTTTTTACAAAGATAGATCGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATT
ATTTTGCTATATGCAGCCATGTTACTCCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

83.136

99.705

0.829
  comGB Streptococcus gordonii str. Challis substr. CH1

53.593

98.525

0.528
  comGB/cglB Streptococcus pneumoniae TIGR4

51.343

98.82

0.507
  comGB/cglB Streptococcus pneumoniae Rx1

51.343

98.82

0.507
  comGB/cglB Streptococcus pneumoniae D39

51.343

98.82

0.507
  comGB/cglB Streptococcus pneumoniae R6

51.343

98.82

0.507
  comGB/cglB Streptococcus mitis SK321

51.045

98.82

0.504
  comGB Streptococcus mutans UA140

53.968

92.92

0.501
  comGB Streptococcus mutans UA159

53.651

92.92

0.499
  comGB/cglB Streptococcus mitis NCTC 12261

50.149

98.82

0.496