Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   I8J32_RS01490 Genome accession   NZ_CP071518
Coordinates   332337..333395 (-) Length   352 a.a.
NCBI ID   WP_343225245.1    Uniprot ID   -
Organism   Agrilutibacter solisilvae strain R19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327337..338395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8J32_RS01470 (I8J32_001470) - 328394..330172 (-) 1779 WP_245156466.1 type IV pilus secretin PilQ -
  I8J32_RS01475 (I8J32_001475) - 330343..330894 (-) 552 WP_200614345.1 pilus assembly protein PilP -
  I8J32_RS01480 (I8J32_001480) - 330896..331606 (-) 711 WP_200614346.1 type 4a pilus biogenesis protein PilO -
  I8J32_RS01485 (I8J32_001485) - 331603..332337 (-) 735 WP_200614348.1 PilN domain-containing protein -
  I8J32_RS01490 (I8J32_001490) pilM 332337..333395 (-) 1059 WP_343225245.1 pilus assembly protein PilM Machinery gene
  I8J32_RS01495 (I8J32_001495) - 333859..336432 (+) 2574 WP_200614351.1 penicillin-binding protein 1A -
  I8J32_RS01500 (I8J32_001500) - 336639..337304 (+) 666 WP_200614352.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37757.16 Da        Isoelectric Point: 4.5078

>NTDB_id=545907 I8J32_RS01490 WP_343225245.1 332337..333395(-) (pilM) [Agrilutibacter solisilvae strain R19]
MGIVTKSQPALVGVDISSTAVKLLQLSRAGDRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAVNRSGTKVKFAAAA
VAGSSVITKVIPMPADLDEDDLESQVELEAVNYVPYPIEEVNLDFEVLGPMPNNPGTLQVLLAASRSENVELRSSALEIG
GLTPRVMDVEAFAIENAFSLLANQLSGPRDGLTALIDSGATMTTLNVLRTGRSLYSREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYANEVLEPFKEAMVQQVSRLLQFFYAGSEFNRVDQIVLAGGNAAIPGIAQMVEEQLGVPTLVANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=545907 I8J32_RS01490 WP_343225245.1 332337..333395(-) (pilM) [Agrilutibacter solisilvae strain R19]
GTGGGGATAGTCACAAAAAGCCAGCCGGCCCTCGTCGGCGTGGACATCAGCTCGACGGCCGTCAAGCTGTTGCAGCTCTC
CCGTGCGGGCGACCGCTACCGGGTGGAGCACTACGCGGTCGAGCCGCTGCCGCCCAATGCGGTGGTCGAAAAGAACATCG
TCGAGGTCGAGGCGGTGGGCGAGGCCATCCGGCGCGCGGTCAACCGCTCGGGGACCAAGGTCAAGTTCGCCGCGGCAGCG
GTCGCGGGGTCCTCGGTGATCACCAAAGTGATCCCGATGCCAGCCGATCTCGACGAGGACGACCTGGAATCCCAGGTTGA
ACTGGAAGCGGTCAACTACGTCCCGTACCCGATCGAGGAAGTGAACCTCGATTTCGAGGTGCTGGGTCCCATGCCGAACA
ATCCCGGCACGCTCCAGGTCCTGCTGGCCGCCTCGCGGTCGGAGAACGTGGAGCTGCGGTCCTCCGCGCTCGAGATCGGC
GGGCTCACCCCGCGGGTCATGGACGTGGAGGCCTTCGCGATCGAGAACGCCTTCTCGCTGCTGGCCAACCAGCTCAGCGG
GCCGCGCGACGGCCTGACCGCGCTGATCGACTCCGGCGCGACGATGACCACGCTCAATGTCCTGCGTACGGGCCGCAGCC
TGTACAGCCGCGAACAGGTATTCGGCGGCAAGCAGCTGACCGACGAGGTCATGCGGCGCTACGGGCTCAGCTACGAGGAA
GCCGGACTGGCCAAGCGCCAGGGCGGCCTGCCCGAGAGTTACGCCAACGAAGTGCTGGAGCCGTTCAAGGAAGCGATGGT
CCAGCAGGTCAGCCGACTGTTGCAGTTCTTCTATGCCGGCAGTGAATTCAACCGGGTCGACCAGATCGTCCTGGCCGGCG
GCAACGCGGCCATCCCGGGCATCGCGCAGATGGTCGAGGAGCAGCTGGGCGTGCCCACCCTGGTGGCCAACCCGCTGGCC
CACATGACGCTCGGTCCGCGCGTGCAGGCGCACGCGCTGGCGCAGGACGCGCCCGCCCTGATGATTGCCTGCGGCCTGGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.009

98.58

0.503
  comM Acinetobacter nosocomialis M2

51.009

98.58

0.503
  comM Acinetobacter baylyi ADP1

49.856

98.58

0.491
  pilM Legionella pneumophila strain ERS1305867

44.477

97.727

0.435