Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LO008_RS01705 Genome accession   NZ_CP087351
Coordinates   356950..357987 (-) Length   345 a.a.
NCBI ID   WP_000355491.1    Uniprot ID   A0A7U3Y5S0
Organism   Acinetobacter baumannii strain DB003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 351950..362987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LO008_RS01675 - 352734..353189 (+) 456 WP_000782976.1 bacteriohemerythrin -
  LO008_RS01680 - 353342..354409 (+) 1068 WP_000092403.1 hypothetical protein -
  LO008_RS01685 - 354409..354732 (+) 324 WP_000442585.1 RnfH family protein -
  LO008_RS01690 - 354787..355185 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  LO008_RS01695 fur 355297..355734 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  LO008_RS01700 pilU 355804..356922 (-) 1119 WP_000347036.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LO008_RS01705 pilT 356950..357987 (-) 1038 WP_000355491.1 type IV pilus twitching motility protein PilT Machinery gene
  LO008_RS01710 - 358114..358806 (+) 693 WP_001108506.1 YggS family pyridoxal phosphate-dependent enzyme -
  LO008_RS01715 - 359008..362604 (-) 3597 WP_072292252.1 AAA family ATPase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38254.88 Da        Isoelectric Point: 6.8886

>NTDB_id=545683 LO008_RS01705 WP_000355491.1 356950..357987(-) (pilT) [Acinetobacter baumannii strain DB003]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICGYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=545683 LO008_RS01705 WP_000355491.1 356950..357987(-) (pilT) [Acinetobacter baumannii strain DB003]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTCTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCTCTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAACGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCTGGTGCAGTCTTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTTTAGGGCAAATCTTTAAAGATATTTGCGGCTATCCTCGCGGCATTGTACTGGTGACGGGACCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCCCTTCGCTCAGCACTGCGTGAAGACCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACCATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACACTCCACACCACGTCAGCTGCAAAAACTATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCAGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGTGGTGGCCGTGTCGCAGCGCATGAGATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCGCAAATGTACTCAGCCATTCAAACTGGTGCCAATCATGGCATGACCACACTCGACCAAAGTTTA
AAAGGCTTAGTCGCTCGCGGTGTAATTAGCCCGCAAACGGCTCGTACTGCAGCAAAACAGCCTGAATCATTCTTATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U3Y5S0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

99.71

100

0.997
  pilT Acinetobacter baumannii D1279779

99.71

100

0.997
  pilT Acinetobacter nosocomialis M2

99.42

100

0.994
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699
  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388