Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   J0J32_RS02025 Genome accession   NZ_CP071285
Coordinates   421301..423511 (+) Length   736 a.a.
NCBI ID   WP_017370462.1    Uniprot ID   -
Organism   Lactococcus garvieae strain LG728     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416301..428511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J32_RS02010 (J0J32_02010) - 417676..419232 (+) 1557 WP_004259192.1 ABC-F family ATP-binding cassette domain-containing protein -
  J0J32_RS02015 (J0J32_02015) - 419398..420573 (+) 1176 WP_004259189.1 SLC13 family permease -
  J0J32_RS02020 (J0J32_02020) - 420631..421281 (+) 651 WP_004259187.1 helix-hairpin-helix domain-containing protein -
  J0J32_RS02025 (J0J32_02025) comEC 421301..423511 (+) 2211 WP_017370462.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  J0J32_RS02030 (J0J32_02030) - 423666..424442 (+) 777 WP_004259182.1 alpha/beta hydrolase family protein -
  J0J32_RS02035 (J0J32_02035) - 424638..424853 (+) 216 WP_003135074.1 F0F1 ATP synthase subunit C -
  J0J32_RS02040 (J0J32_02040) atpB 424908..425621 (+) 714 WP_004259176.1 F0F1 ATP synthase subunit A -
  J0J32_RS02045 (J0J32_02045) atpF 425635..426141 (+) 507 WP_004259173.1 F0F1 ATP synthase subunit B -
  J0J32_RS02050 (J0J32_02050) - 426156..426683 (+) 528 WP_014024339.1 F0F1 ATP synthase subunit delta -
  J0J32_RS02055 (J0J32_02055) atpA 426749..428257 (+) 1509 WP_004259170.1 F0F1 ATP synthase subunit alpha -

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 85388.16 Da        Isoelectric Point: 9.7314

>NTDB_id=544579 J0J32_RS02025 WP_017370462.1 421301..423511(+) (comEC) [Lactococcus garvieae strain LG728]
MKQKFSLIYFVYLLVFVYFLIFSFSWPLLILSLFSFFIVFYRQYYSVLVLLLCFSLFLLMIKKETEVQERKQPEKISKIT
PYMDTLQVNGSRLSFRGEVNGNDFQAFYTLTSEKEKEYFKKLAVKGTFYFEGTLEIPEEQRNFSGFDYRKYLRTQGVYRL
LKIEKIHGFEEKQDFDLRLVRRKAILWAQEYFPSPMSSYMTGLLFGWLDKDFEEMGELYTSLGIIHLFALSGMQVNFFIE
LLRNILLRLGLSQKLVFFIQIPFSIFYAFLTGLSLSILRALLQKNIPLKNSSDRFSVTFLLLMVFSPYFLLTTGGQLTML
YSFLITFFSRKFKELKGLKKSLLDSTVLAVGVLPLLIFHFHSFQPLSIFLTLLFSLIFDFLLLPGLLFVFLLSLFGTALT
LFNPFFIVLESIVRWVGDLFDQPIVFGKPNLIFLLLLFMLSGLALDFYKKRNWNLSIIIILFLLFFFVKNPKHPSITMVD
VRQGDSILLQDSWNSQNILIDTGGRLKIRADEQWKEGIYKSNAENTLLPYLRARGVSKIDVLIVTHPDEDHIGDLESVAQ
KVPIKNVYVSASALEKKSFAKKLSRLNSQIHIVQEGDKLPIFNSYLWFLTTGVEGKSDNDNSLVTFGEFYGKKFLFTGDL
EEEGEVRLQEEYPHLEVDVLKVGHHGSKTSSKEAFLEAINPKLALISAGKNNRYKHPNQETLQQFAARGIKIYRTDQQGA
IRLLYKKGSWHIQTVK

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=544579 J0J32_RS02025 WP_017370462.1 421301..423511(+) (comEC) [Lactococcus garvieae strain LG728]
ATGAAACAAAAATTTTCGCTTATCTATTTCGTTTATCTCTTAGTCTTTGTTTATTTTCTTATTTTTAGTTTTTCATGGCC
TTTACTTATTCTTAGTCTCTTTAGTTTCTTCATCGTTTTTTATCGTCAATACTATAGTGTGTTAGTTCTTTTACTGTGCT
TTAGTTTATTCTTACTCATGATAAAAAAAGAAACGGAAGTACAAGAAAGAAAGCAACCAGAAAAAATTTCGAAAATTACC
CCTTATATGGATACTCTTCAAGTAAATGGAAGCAGGCTCAGTTTCAGAGGAGAAGTGAACGGCAACGACTTCCAAGCTTT
TTACACCTTGACCTCGGAAAAAGAAAAAGAATACTTCAAAAAACTTGCGGTGAAGGGGACTTTTTACTTTGAAGGCACTT
TGGAGATTCCAGAAGAGCAAAGAAACTTTTCAGGTTTTGATTACAGAAAGTATTTAAGGACTCAAGGGGTCTATCGTCTT
CTTAAAATAGAAAAAATTCATGGCTTTGAAGAAAAACAGGACTTTGATTTACGGCTGGTACGCCGAAAAGCCATTCTTTG
GGCTCAGGAATATTTCCCTTCTCCTATGTCGTCCTATATGACAGGGCTTCTTTTTGGATGGTTGGATAAAGACTTTGAAG
AAATGGGGGAGCTTTACACCAGTCTTGGCATTATCCATTTATTTGCGCTGTCAGGCATGCAGGTGAATTTTTTTATTGAA
CTTCTCCGAAATATTTTACTGCGGCTTGGCTTATCACAGAAGTTAGTCTTTTTTATCCAAATTCCCTTTTCGATATTTTA
TGCATTTCTTACAGGCTTATCTCTTTCAATCTTACGCGCGCTTCTTCAAAAAAATATCCCCCTTAAAAATTCAAGCGATA
GATTTTCTGTCACTTTTCTTCTTTTAATGGTGTTCAGTCCTTATTTTCTGCTGACAACAGGAGGACAACTCACGATGCTT
TATTCTTTCTTAATCACATTTTTTTCACGAAAGTTCAAAGAACTAAAAGGATTAAAAAAATCGTTACTTGATTCTACCGT
CCTAGCTGTTGGGGTTCTTCCGCTTTTGATTTTTCACTTTCATAGTTTTCAGCCATTATCGATTTTCTTGACTTTATTAT
TTAGTCTGATTTTTGATTTTTTACTTCTGCCAGGTTTACTTTTTGTTTTTCTTCTTTCTTTATTCGGTACTGCTCTCACA
TTATTTAATCCTTTTTTTATTGTTTTAGAATCAATAGTAAGATGGGTGGGCGATTTATTTGACCAACCAATTGTCTTTGG
CAAACCCAACCTTATTTTTTTACTTTTACTTTTTATGCTGAGTGGACTTGCCCTTGATTTTTATAAAAAAAGAAACTGGA
ACTTGAGCATAATAATTATTCTTTTCTTGCTTTTTTTCTTTGTTAAGAATCCTAAACACCCAAGCATTACAATGGTAGAT
GTGAGACAAGGGGATAGTATTCTTTTACAAGACAGTTGGAACAGCCAAAACATCCTCATCGATACGGGCGGGAGATTAAA
AATAAGAGCTGATGAGCAGTGGAAGGAGGGAATCTATAAGAGCAATGCCGAAAATACACTTCTGCCTTATCTGAGAGCGC
GTGGCGTGAGTAAGATAGATGTTCTGATTGTGACACATCCAGATGAGGATCATATTGGAGACCTGGAGAGTGTAGCTCAG
AAAGTACCAATAAAAAATGTTTATGTGTCAGCTTCTGCATTGGAGAAGAAAAGCTTTGCTAAAAAACTCAGCCGTTTAAA
CAGTCAGATTCACATTGTGCAGGAGGGGGACAAGCTCCCGATCTTTAACAGTTATCTCTGGTTTTTAACGACAGGCGTTG
AAGGTAAAAGCGACAATGATAATTCTTTAGTGACATTTGGTGAATTTTATGGGAAGAAATTTCTTTTTACAGGTGACTTA
GAAGAAGAGGGAGAGGTGCGGCTCCAGGAAGAATATCCCCATTTAGAGGTGGATGTCCTAAAAGTTGGACATCATGGCAG
TAAAACTTCTTCAAAGGAAGCTTTTTTAGAAGCGATCAACCCTAAGCTCGCTCTTATTTCGGCTGGAAAGAATAATCGAT
ATAAACACCCAAATCAAGAAACGCTCCAACAATTCGCTGCACGAGGCATAAAAATTTATCGTACAGACCAGCAAGGGGCA
ATCCGTCTCCTTTATAAAAAAGGCAGTTGGCACATTCAAACAGTAAAGTAG

Domains

Predicted by InterproScan.

Competence protein (Competence)

(203-445)

Metallo-beta-lactamase superfamily (Lactamase_B)

(481-689)

Domain of unknown function (DUF4131) (DUF4131)

(25-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Lactococcus lactis subsp. cremoris KW2

52.66

99.592

0.524
  comEC/celB Streptococcus mitis SK321

44.98

100

0.457
  comEC/celB Streptococcus mitis NCTC 12261

43.548

100

0.44
  comEC/celB Streptococcus pneumoniae TIGR4

43.203

100

0.436
  comEC/celB Streptococcus pneumoniae Rx1

42.799

100

0.432
  comEC/celB Streptococcus pneumoniae D39

42.799

100

0.432
  comEC/celB Streptococcus pneumoniae R6

42.799

100

0.432