Detailed information NTG0054407

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: COA_RS0108345
• Genome accession: NZ_AKBV01000001
• Coordinates: 1714517..1715527 (-)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1709517..1720527

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
COA_RS0108325	mepM	1710484..1711806 (-)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-
COA_RS0108330	znuA	1711822..1712754 (-)	933	WP_001300644.1	zinc ABC transporter substrate-binding protein ZnuA	-
COA_RS0108335	znuC	1712833..1713588 (+)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
COA_RS0108340	znuB	1713585..1714370 (+)	786	WP_000571480.1	zinc ABC transporter permease subunit ZnuB	-
COA_RS0108345	ruvB	1714517..1715527 (-)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
COA_RS0108350	ruvA	1715536..1716147 (-)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
COA_RS1000000124855	yobI	1716286..1716351 (-)	66	WP_010723105.1	stress response small protein YobI	-
COA_RS0108355	yebB	1716422..1717024 (+)	603	WP_001024932.1	YebB family permuted papain-like enzyme	-
COA_RS0108360	ruvC	1717026..1717547 (-)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
COA_RS0108365	yebC	1717582..1718322 (-)	741	WP_000907248.1	YebC/PmpR family DNA-binding transcriptional regulator	-
COA_RS0108370	nudB	1718351..1718803 (-)	453	WP_001300367.1	dihydroneopterin triphosphate diphosphatase	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=54407 COA_RS0108345 WP_000568519.1 1714517..1715527(-) (ruvB) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=54407 COA_RS0108345 WP_000568519.1 1714517..1715527(-) (ruvB) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCCAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGACCTGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494