Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M5M_RS05700 Genome accession   NC_018868
Coordinates   1256868..1258049 (+) Length   393 a.a.
NCBI ID   WP_015046518.1    Uniprot ID   K4KJG3
Organism   Simiduia agarivorans SA1 = DSM 21679     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1251868..1263049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5M_RS05685 (M5M_05685) - 1252508..1252744 (-) 237 WP_016389236.1 DUF2789 domain-containing protein -
  M5M_RS05690 (M5M_05690) - 1252964..1253506 (+) 543 WP_015046516.1 porin family protein -
  M5M_RS05695 (M5M_05695) - 1253566..1256541 (-) 2976 WP_015046517.1 TonB-dependent receptor -
  M5M_RS05700 (M5M_05700) pilU 1256868..1258049 (+) 1182 WP_015046518.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5M_RS05705 (M5M_05705) - 1258248..1258796 (+) 549 WP_016389237.1 outer membrane beta-barrel protein -
  M5M_RS05710 (M5M_05710) - 1258860..1259189 (-) 330 WP_015046520.1 hypothetical protein -
  M5M_RS05715 (M5M_05715) - 1259329..1261485 (-) 2157 WP_015046521.1 S9 family peptidase -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 43719.68 Da        Isoelectric Point: 5.9847

>NTDB_id=54082 M5M_RS05700 WP_015046518.1 1256868..1258049(+) (pilU) [Simiduia agarivorans SA1 = DSM 21679]
MTTPIDNYLNILAKKDGSDLYLSAGAPPCAKFQGQLKPLSQTPYKSGEIDAIAQALMDDAQRAVFEEELEMNLAVSIAGV
GRFRINIFKQRNEVSIVARNINTEIPKFENLGLPEVLKEVIMTKRGLVLFVGGTGSGKSTSLAALIDHRNSNSGGHIITI
EDPVEYVHRHKKSIINQREVGVDTRSFRNALKNTLRQAPDVILIGEIRDRETMEHALEFADTGHLAISTLHANNANQALE
RIINFFPEERRPQLLLSLSQNLRAFVSQRLIPTIDGKRCAAVEVLLGTKTIQEMIYQSRFHEIKEIMEKSENLGMQTFDG
ALFHLYVAGKISHDNAIANADSANNLRLRIKLHEKGEASTAPKPQEQSSQESKPLSFGNLTLEAMDEDEDEPE

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=54082 M5M_RS05700 WP_015046518.1 1256868..1258049(+) (pilU) [Simiduia agarivorans SA1 = DSM 21679]
ATGACTACCCCAATAGATAACTACCTCAACATCCTCGCCAAGAAAGACGGCTCAGATCTTTATCTGAGTGCTGGCGCCCC
ACCCTGTGCCAAATTCCAGGGGCAACTCAAACCCCTGAGCCAGACCCCGTACAAATCCGGTGAAATTGACGCCATCGCCC
AGGCCCTGATGGATGATGCCCAACGGGCGGTATTCGAAGAAGAACTGGAAATGAATCTGGCGGTCTCCATTGCCGGCGTC
GGCCGCTTTCGCATCAATATTTTCAAGCAACGCAACGAGGTGTCTATCGTTGCCCGCAACATCAATACAGAAATACCCAA
ATTCGAAAATCTTGGGCTGCCCGAGGTATTGAAAGAAGTCATCATGACCAAGCGCGGACTGGTACTGTTTGTGGGCGGGA
CCGGCTCGGGCAAATCCACCAGCCTTGCGGCGTTGATTGATCACCGCAACAGCAATTCCGGCGGCCACATCATCACGATT
GAAGATCCGGTGGAATACGTTCACCGCCATAAAAAAAGCATCATCAACCAGCGCGAAGTGGGCGTTGATACCCGCAGTTT
TCGCAATGCCCTGAAAAACACGCTGCGCCAGGCGCCGGATGTCATCCTGATCGGTGAAATCCGGGACCGCGAGACCATGG
AACACGCCCTTGAATTTGCCGACACCGGCCATCTGGCCATTTCGACGTTACATGCCAACAACGCCAATCAGGCGCTCGAG
CGCATTATTAATTTTTTCCCCGAAGAACGGCGCCCGCAATTACTGCTGTCGCTGTCACAGAATCTGCGCGCCTTTGTTTC
CCAGCGCTTGATCCCCACCATCGACGGCAAACGCTGTGCGGCCGTTGAAGTATTGCTCGGCACCAAAACCATCCAGGAAA
TGATCTACCAATCCCGCTTCCATGAAATCAAAGAAATCATGGAGAAATCCGAAAACCTGGGCATGCAAACCTTTGATGGC
GCGCTTTTTCACCTTTACGTCGCCGGCAAAATTTCACACGACAATGCCATTGCCAATGCCGACTCCGCCAACAACCTGAG
ATTACGGATCAAATTGCATGAGAAAGGCGAAGCCTCCACTGCACCAAAACCGCAAGAGCAATCAAGTCAAGAAAGCAAGC
CGCTCAGCTTTGGCAACCTGACGCTGGAGGCGATGGATGAAGACGAGGACGAGCCCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(82-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K4KJG3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.208

97.964

0.511
  pilU Acinetobacter baylyi ADP1

51.2

95.42

0.489
  pilU Vibrio cholerae strain A1552

50.575

88.55

0.448
  pilT Pseudomonas aeruginosa PAK

41.52

87.023

0.361

Multiple sequence alignment