Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   I6J48_RS10720 Genome accession   NZ_CP070518
Coordinates   2280214..2280654 (+) Length   146 a.a.
NCBI ID   WP_205468447.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus strain FDAARGOS_1218     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2275214..2285654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J48_RS10700 (I6J48_10700) - 2276602..2277489 (+) 888 WP_205468444.1 metal-dependent hydrolase -
  I6J48_RS10705 (I6J48_10705) - 2277618..2278208 (+) 591 WP_005044023.1 LemA family protein -
  I6J48_RS10710 (I6J48_10710) - 2278233..2279312 (+) 1080 WP_205468445.1 YgcG family protein -
  I6J48_RS10715 (I6J48_10715) - 2279306..2279866 (+) 561 WP_205468446.1 TPM domain-containing protein -
  I6J48_RS10720 (I6J48_10720) pilA 2280214..2280654 (+) 441 WP_205468447.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  I6J48_RS10725 (I6J48_10725) - 2280712..2282025 (+) 1314 WP_205468448.1 O-antigen ligase -
  I6J48_RS10730 (I6J48_10730) - 2282131..2283759 (+) 1629 WP_205468449.1 PglL family O-oligosaccharyltransferase -
  I6J48_RS10735 (I6J48_10735) bfr 2283803..2284267 (-) 465 WP_005038304.1 bacterioferritin -
  I6J48_RS10740 (I6J48_10740) - 2284513..2284707 (-) 195 WP_004639703.1 bacterioferritin-associated ferredoxin -
  I6J48_RS10745 (I6J48_10745) - 2284927..2285310 (-) 384 WP_002116996.1 RidA family protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15262.22 Da        Isoelectric Point: 4.2682

>NTDB_id=540493 I6J48_RS10720 WP_205468447.1 2280214..2280654(+) (pilA) [Acinetobacter calcoaceticus strain FDAARGOS_1218]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIAKSQVAEPFTLIDGAKASIQLNLEKNSCTKVSGKDDTVTGKYGVLT
ISGEPDADAAEDADSGCTVSYEFNATDVSSKVASKTIAAHIYNNGTLVDNGTTVDPELLPKSFNAS

Nucleotide


Download         Length: 441 bp        

>NTDB_id=540493 I6J48_RS10720 WP_205468447.1 2280214..2280654(+) (pilA) [Acinetobacter calcoaceticus strain FDAARGOS_1218]
ATGAATGCACAAAAAGGTTTTACATTAATTGAACTTATGATCGTTGTTGCGATTATTGGTATTTTGGCGGCAATTGCGAT
TCCTGCATATCAAAACTATATTGCTAAGTCGCAGGTTGCGGAACCATTTACACTAATTGATGGTGCTAAAGCATCGATTC
AATTGAACTTAGAGAAAAATTCATGTACTAAAGTATCTGGTAAGGATGACACAGTAACTGGTAAGTATGGAGTGTTAACG
ATTTCTGGTGAACCTGATGCAGATGCAGCAGAAGATGCAGATTCTGGCTGTACAGTATCTTATGAATTCAATGCTACGGA
TGTTTCTTCAAAAGTAGCAAGTAAAACTATCGCTGCCCATATTTATAATAATGGTACATTAGTTGACAATGGCACTACTG
TAGATCCAGAATTATTACCTAAATCATTCAATGCTTCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter nosocomialis M2

52.98

100

0.548
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.411

100

0.404
  pilA Vibrio cholerae strain A1552

40.411

100

0.404
  pilA Vibrio cholerae C6706

40.411

100

0.404