Detailed information NTG0540192

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: JWZ98_RS01250
• Genome accession: NZ_CP070494
• Coordinates: 276497..277558 (+)
• Length: 353 a.a.
• NCBI ID: WP_020483743.1
• Uniprot ID: A0A2N0V797
• Organism: Methylomonas sp. EFPC1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 271497..282558

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JWZ98_RS01230 (JWZ98_01230)	-	271712..271921 (-)	210	WP_205452709.1	hypothetical protein	-
JWZ98_RS01235 (JWZ98_01235)	-	272050..273357 (+)	1308	WP_240348168.1	MFS transporter	-
JWZ98_RS01240 (JWZ98_01240)	rpmE	273651..273851 (+)	201	WP_081607938.1	50S ribosomal protein L31	-
JWZ98_RS01245 (JWZ98_01245)	-	273923..276286 (-)	2364	WP_205452711.1	penicillin-binding protein 1A	-
JWZ98_RS01250 (JWZ98_01250)	pilM	276497..277558 (+)	1062	WP_020483743.1	pilus assembly protein PilM	Machinery gene
JWZ98_RS01255 (JWZ98_01255)	-	277558..278130 (+)	573	WP_205452712.1	PilN domain-containing protein	-
JWZ98_RS01260 (JWZ98_01260)	-	278127..278753 (+)	627	WP_033156771.1	type 4a pilus biogenesis protein PilO	-
JWZ98_RS01265 (JWZ98_01265)	-	278750..279307 (+)	558	WP_084153287.1	pilus assembly protein PilP	-
JWZ98_RS01270 (JWZ98_01270)	pilQ	279310..281553 (+)	2244	WP_033156770.1	type IV pilus secretin PilQ	-
JWZ98_RS01275 (JWZ98_01275)	-	281695..281820 (+)	126	WP_084153286.1	lipoprotein	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 37937.42 Da
• Isoelectric Point: 4.7094

>NTDB_id=540192 JWZ98_RS01250 WP_020483743.1 276497..277558(+) (pilM) [Methylomonas sp. EFPC1]
MSWLSRNPSAVLGVDISTAAVKLLELSRVGARYRVESYSVAPLPQDAIVDKNITNIEQIGAVIKAAVKQSGTRAKQASVA
VAGSSVMTKIISMPASLSDRDMEEQIMVEADQYIPYSLDEVNLDFEVQGITKNNPDMVDVLLAASRRENIEDRVAALAYA
GLKAVVVDVEAFAMENAFSLLADQLPEGAGNMTVAIADIGATMTSLNILHEGKTVYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKKHGGLPDNYVTDVLEPFKKAMLQQIARSLQFFVSSSANRGVDALVLAGGCASIAGLDKLVERDLGIPSYIANPF
INMALSNRVKPQSLSNDTPAMMIACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=540192 JWZ98_RS01250 WP_020483743.1 276497..277558(+) (pilM) [Methylomonas sp. EFPC1]
ATGAGCTGGTTGAGCAGGAATCCGTCGGCTGTGCTGGGTGTGGATATTAGTACAGCTGCGGTTAAATTGTTGGAATTGAG
CCGGGTCGGTGCTCGTTACCGGGTAGAAAGTTACTCAGTGGCGCCCTTACCTCAAGATGCGATAGTCGATAAGAACATCA
CCAATATAGAGCAAATCGGCGCGGTAATAAAAGCGGCGGTCAAGCAATCCGGCACTCGGGCAAAGCAGGCGAGCGTTGCG
GTTGCCGGTTCCTCGGTGATGACCAAAATCATTTCGATGCCCGCCTCCTTGTCAGACAGGGACATGGAGGAGCAGATCAT
GGTGGAAGCGGATCAGTACATTCCTTATTCGCTGGACGAGGTTAATCTCGATTTCGAAGTCCAGGGCATCACCAAAAATA
ACCCGGATATGGTTGATGTGTTGCTGGCGGCTTCTCGGCGGGAAAACATTGAAGACAGGGTGGCTGCACTGGCCTATGCC
GGCCTTAAGGCTGTCGTCGTCGATGTGGAAGCTTTCGCGATGGAAAATGCGTTTTCGTTGTTGGCGGATCAGTTACCGGA
AGGTGCCGGCAATATGACTGTTGCGATCGCCGACATTGGCGCAACCATGACCTCTCTGAACATTTTGCATGAAGGTAAAA
CTGTTTATACCCGGGAGCAGGGCTTTGGCGGAAAACAGCTAACTGAAGAGATTCAGCGCCGCTATGGCCTGTCGTATGAA
GAAGCCGGTTTGGCCAAAAAGCACGGCGGCTTGCCGGACAATTACGTCACGGATGTGCTGGAGCCCTTCAAAAAAGCCAT
GTTGCAGCAAATCGCCAGATCGTTGCAATTCTTTGTTTCCTCTAGCGCCAATCGCGGCGTCGATGCCTTGGTACTTGCCG
GTGGTTGCGCGTCAATCGCGGGTTTGGACAAATTGGTGGAACGCGACCTCGGCATCCCGTCTTACATTGCCAATCCATTT
ATCAATATGGCGCTTTCTAATCGGGTCAAACCGCAAAGTCTGAGCAACGATACGCCTGCGATGATGATAGCGTGCGGTTT
AGCGTTAAGGAGTTTCGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

3D structure

• AlphaFold DB: A0A2N0V797

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.695	100	0.518
comM	Acinetobacter nosocomialis M2	51.695	100	0.518
comM	Acinetobacter baylyi ADP1	50	100	0.501
pilM	Legionella pneumophila strain ERS1305867	40.793	100	0.408