Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   JWZ97_RS13310 Genome accession   NZ_CP070491
Coordinates   3109158..3110219 (-) Length   353 a.a.
NCBI ID   WP_205430043.1    Uniprot ID   -
Organism   Methylococcus sp. EFPC2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3104158..3115219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWZ97_RS13285 (JWZ97_13285) aroK 3104434..3104997 (-) 564 WP_205430036.1 shikimate kinase AroK -
  JWZ97_RS13290 (JWZ97_13290) pilQ 3105118..3107370 (-) 2253 WP_240342346.1 type IV pilus secretin PilQ -
  JWZ97_RS13295 (JWZ97_13295) - 3107367..3107933 (-) 567 WP_205430038.1 pilus assembly protein PilP -
  JWZ97_RS13300 (JWZ97_13300) - 3107942..3108577 (-) 636 WP_205430039.1 type 4a pilus biogenesis protein PilO -
  JWZ97_RS13305 (JWZ97_13305) - 3108574..3109158 (-) 585 WP_205430041.1 PilN domain-containing protein -
  JWZ97_RS13310 (JWZ97_13310) pilM 3109158..3110219 (-) 1062 WP_205430043.1 pilus assembly protein PilM Machinery gene
  JWZ97_RS13315 (JWZ97_13315) - 3110480..3112897 (+) 2418 WP_205430045.1 penicillin-binding protein 1A -
  JWZ97_RS13320 (JWZ97_13320) - 3112889..3113662 (-) 774 WP_205430047.1 zinc-dependent peptidase -
  JWZ97_RS13325 (JWZ97_13325) - 3113769..3114158 (+) 390 WP_205430048.1 DUF423 domain-containing protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38093.73 Da        Isoelectric Point: 4.8320

>NTDB_id=540186 JWZ97_RS13310 WP_205430043.1 3109158..3110219(-) (pilM) [Methylococcus sp. EFPC2]
MFFLQRKKPMLLGIDISTAAVKLLELGKAGPRYRVESYAVIPLPQDAVVDKTIAKVEVIGNAVKSVVAQSGTKLKHAAVA
VASSSVISKVITMPAALSDGELESQIELEADQYIPYPLDEISMDFEVQGSNEKNPEMIDVLLVASRKENVEDRVEALEFA
GLKAEILDVESYALENAFALILDQLSDANPGKTIAIADIGATMTTLNVLHNGRIIYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKKHGGLPDNYLPEVLEPFKHAMAQQIQRSLQFFASSSTHRGVEGLVLAGGCASIAGVERFVEQTTGIPAVVANPF
INMTLSSRVKPQALSNDAPAMMTACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=540186 JWZ97_RS13310 WP_205430043.1 3109158..3110219(-) (pilM) [Methylococcus sp. EFPC2]
ATGTTTTTTCTCCAGCGCAAGAAACCGATGTTGCTGGGTATCGACATCAGCACGGCAGCGGTTAAGTTGCTGGAGCTCGG
CAAGGCGGGACCCCGCTATCGGGTGGAAAGCTACGCTGTCATACCCTTGCCGCAGGACGCCGTGGTCGACAAGACCATCG
CCAAGGTGGAAGTCATAGGCAACGCGGTCAAGTCCGTGGTGGCGCAATCCGGAACCAAGCTCAAGCATGCCGCGGTGGCC
GTCGCCAGCTCGTCGGTCATCAGCAAGGTCATCACGATGCCCGCGGCATTGAGCGACGGCGAACTGGAATCGCAAATCGA
GCTCGAAGCCGACCAATATATTCCCTACCCTCTCGACGAAATCAGCATGGATTTCGAGGTGCAGGGCTCGAACGAGAAAA
ATCCGGAAATGATCGATGTGCTCCTGGTCGCATCCCGGAAGGAGAACGTCGAAGACCGGGTCGAGGCGCTGGAGTTTGCC
GGTTTGAAGGCGGAAATTCTGGATGTCGAATCCTATGCCTTAGAAAACGCGTTTGCCCTGATCCTCGATCAGCTCAGCGA
TGCTAATCCGGGAAAAACAATCGCCATCGCCGACATCGGAGCCACGATGACGACGCTGAATGTCCTGCACAACGGGCGCA
TCATTTATACCCGTGAGCAAGGATTCGGCGGGAAACAGCTGACCGAGGAAATCCAGCGCCGTTACGGGCTGTCCTACGAA
GAGGCCGGCCTGGCCAAGAAGCATGGCGGATTGCCGGACAACTATCTGCCCGAGGTGCTGGAACCGTTCAAGCATGCCAT
GGCCCAGCAGATTCAGCGCTCCTTGCAGTTCTTCGCCTCGTCGAGCACGCATCGGGGCGTCGAAGGCCTGGTGCTGGCAG
GCGGATGTGCGTCGATCGCCGGCGTCGAGCGCTTCGTGGAGCAAACGACCGGGATACCCGCCGTAGTCGCCAACCCCTTT
ATCAATATGACCCTCTCCAGCCGGGTAAAGCCGCAGGCGCTCAGTAACGACGCCCCGGCGATGATGACGGCTTGCGGGTT
GGCGCTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.542

100

0.527
  comM Acinetobacter nosocomialis M2

52.26

100

0.524
  comM Acinetobacter baylyi ADP1

48.997

98.867

0.484
  pilM Legionella pneumophila strain ERS1305867

43.875

99.433

0.436