Detailed information NTG0539393

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JWB57_RS12925
• Genome accession: NZ_CP070358
• Coordinates: 2616584..2617702 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain AB5075-VUB-itrA::ISAba13
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2611584..2622702

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JWB57_RS12915 (JWB57_14920)	-	2614700..2615392 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
JWB57_RS12920 (JWB57_14925)	pilT	2615519..2616556 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
JWB57_RS12925 (JWB57_14930)	pilU	2616584..2617702 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
JWB57_RS12930 (JWB57_14935)	fur	2617772..2618209 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
JWB57_RS12935 (JWB57_14940)	bamE	2618321..2618719 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
JWB57_RS12940 (JWB57_14945)	-	2618774..2619097 (-)	324	WP_000442585.1	RnfH family protein	-
JWB57_RS12945 (JWB57_14950)	-	2619097..2620164 (-)	1068	WP_000092403.1	hypothetical protein	-
JWB57_RS12950 (JWB57_14955)	-	2620317..2620772 (-)	456	WP_000782976.1	bacteriohemerythrin	-
JWB57_RS12955 (JWB57_14960)	-	2621025..2621873 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=539393 JWB57_RS12925 WP_000347036.1 2616584..2617702(+) (pilU) [Acinetobacter baumannii strain AB5075-VUB-itrA::ISAba13]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=539393 JWB57_RS12925 WP_000347036.1 2616584..2617702(+) (pilU) [Acinetobacter baumannii strain AB5075-VUB-itrA::ISAba13]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392