Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   D1099_RS05410 Genome accession   NZ_CP069796
Coordinates   1140415..1140861 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae serovar 6 str. Femo     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1135415..1145861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1099_RS05385 (D1099_05350) yacG 1136322..1136510 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1099_RS05390 (D1099_05355) coaE 1136492..1137130 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  D1099_RS05395 (D1099_05360) - 1137152..1137790 (-) 639 WP_005604550.1 prepilin peptidase -
  D1099_RS05400 (D1099_05365) pilC 1137817..1139019 (-) 1203 WP_005608031.1 type II secretion system F family protein Machinery gene
  D1099_RS05405 (D1099_05370) pilB 1139003..1140397 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  D1099_RS05410 (D1099_05375) pilA 1140415..1140861 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1099_RS05415 (D1099_05380) radA 1141043..1142422 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1099_RS05420 (D1099_05385) - 1142466..1142699 (-) 234 WP_005597399.1 YgjV family protein -
  D1099_RS05425 (D1099_05390) folA 1142826..1143314 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1099_RS05430 (D1099_05395) proB 1143401..1144492 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  D1099_RS05435 (D1099_05400) - 1144505..1145281 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=536688 D1099_RS05410 WP_005597397.1 1140415..1140861(-) (pilA) [Actinobacillus pleuropneumoniae serovar 6 str. Femo]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=536688 D1099_RS05410 WP_005597397.1 1140415..1140861(-) (pilA) [Actinobacillus pleuropneumoniae serovar 6 str. Femo]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACGGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527