Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   IUB69_RS02155 Genome accession   NZ_ABACVG020000001
Coordinates   431506..432516 (-) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain 2020CK-00188     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 426506..437516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUB69_RS02135 (IUB69_002130) mepM 427473..428795 (-) 1323 WP_032184424.1 murein DD-endopeptidase MepM -
  IUB69_RS02140 (IUB69_002135) znuA 428811..429743 (-) 933 WP_032184796.1 zinc ABC transporter substrate-binding protein ZnuA -
  IUB69_RS02145 (IUB69_002140) znuC 429822..430577 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  IUB69_RS02150 (IUB69_002145) znuB 430574..431359 (+) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  IUB69_RS02155 (IUB69_002150) ruvB 431506..432516 (-) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  IUB69_RS02160 (IUB69_002155) ruvA 432525..433136 (-) 612 WP_000580328.1 Holliday junction branch migration protein RuvA -
  IUB69_RS02165 (IUB69_002160) - 433343..433888 (-) 546 WP_050487862.1 hypothetical protein -
  IUB69_RS02170 (IUB69_002165) - 433926..434975 (-) 1050 WP_236930346.1 hypothetical protein -
  IUB69_RS02175 (IUB69_002170) - 434883..435446 (+) 564 WP_235841534.1 YebB family permuted papain-like enzyme -
  IUB69_RS02180 (IUB69_002175) ruvC 435448..435969 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  IUB69_RS02185 (IUB69_002180) yebC 436004..436744 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  IUB69_RS02190 (IUB69_002185) nudB 436773..437225 (-) 453 WP_077626310.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=53648 IUB69_RS02155 WP_000568522.1 431506..432516(-) (ruvB) [Escherichia coli strain 2020CK-00188]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=53648 IUB69_RS02155 WP_000568522.1 431506..432516(-) (ruvB) [Escherichia coli strain 2020CK-00188]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAACCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGAGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGTTTAGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCCGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATTATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGACTTGCCGCCGTTTACTCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGCTGCGTGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGTAGTGCCCGTTTTATGGGGCTTGAGATGAGTGACGATGGCGCGCTGGAAGTTGCTC
GCCGGGCGCGTGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGTGTTCGCGATTTTGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCGGATATCGCCGCCCAGGCACTGGATATGCTGAATGTTGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGCCTGGATAACCTGGCGGCAGCCATTGGTGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCACCAGAAATGCCTTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment