NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	I6J89_RS04105	Genome accession	NZ_CP069559
Coordinates	861984..863108 (+)	Length	374 a.a.
NCBI ID	WP_000228517.1	Uniprot ID	E2QFE2
Organism	Escherichia coli strain FDAARGOS_1259
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 856984..868108

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6J89_RS04080 (I6J89_04080)	mscL	857101..857514 (-)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-
I6J89_RS04085 (I6J89_04085)	trkA	857644..859020 (-)	1377	WP_045232965.1	Trk system potassium transporter TrkA	-
I6J89_RS04090 (I6J89_04090)	rsmB	859042..860331 (-)	1290	WP_000744773.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
I6J89_RS04095 (I6J89_04095)	fmt	860383..861330 (-)	948	WP_000004425.1	methionyl-tRNA formyltransferase	-
I6J89_RS04100 (I6J89_04100)	def	861345..861854 (-)	510	WP_000114984.1	peptide deformylase	-
I6J89_RS04105 (I6J89_04105)	dprA	861984..863108 (+)	1125	WP_000228517.1	DNA-protecting protein DprA	Machinery gene
I6J89_RS04110 (I6J89_04110)	smg	863080..863553 (+)	474	WP_000460672.1	DUF494 family protein Smg	-
I6J89_RS04115 (I6J89_04115)	yrdD	863582..864124 (+)	543	WP_001312137.1	type I DNA topoisomerase	-
I6J89_RS04120 (I6J89_04120)	tsaC	864129..864701 (+)	573	WP_001468260.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
I6J89_RS04125 (I6J89_04125)	aroE	864706..865524 (+)	819	WP_000451249.1	shikimate dehydrogenase	-
I6J89_RS04130 (I6J89_04130)	yrdB	865521..865778 (+)	258	WP_001070563.1	DUF1488 domain-containing protein	-
I6J89_RS04135 (I6J89_04135)	yrdA	865754..866308 (-)	555	WP_001286206.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40910.86 Da Isoelectric Point: 6.0395

                                >NTDB_id=535568 I6J89_RS04105 WP_000228517.1 861984..863108(+) (dprA) [Escherichia coli strain FDAARGOS_1259]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=535568 I6J89_RS04105 WP_000228517.1 861984..863108(+) (dprA) [Escherichia coli strain FDAARGOS_1259]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGCCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	E2QFE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0535568