NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	I6J83_RS18385	Genome accession	NZ_CP069549
Coordinates	3856663..3857787 (+)	Length	374 a.a.
NCBI ID	WP_000228508.1	Uniprot ID	A0A0D8VMA7
Organism	Escherichia coli strain FDAARGOS_1253
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 3851663..3862787

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6J83_RS18360 (I6J83_18360)	mscL	3851780..3852193 (-)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-
I6J83_RS18365 (I6J83_18365)	trkA	3852323..3853699 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
I6J83_RS18370 (I6J83_18370)	rsmB	3853721..3855010 (-)	1290	WP_000744764.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
I6J83_RS18375 (I6J83_18375)	fmt	3855062..3856009 (-)	948	WP_000004424.1	methionyl-tRNA formyltransferase	-
I6J83_RS18380 (I6J83_18380)	def	3856024..3856533 (-)	510	WP_000114984.1	peptide deformylase	-
I6J83_RS18385 (I6J83_18385)	dprA	3856663..3857787 (+)	1125	WP_000228508.1	DNA-protecting protein DprA	Machinery gene
I6J83_RS18390 (I6J83_18390)	smg	3857759..3858232 (+)	474	WP_000460673.1	DUF494 family protein Smg	-
I6J83_RS18395 (I6J83_18395)	yrdD	3858261..3858803 (+)	543	WP_001312137.1	type I DNA topoisomerase	-
I6J83_RS18400 (I6J83_18400)	tsaC	3858808..3859380 (+)	573	WP_001296460.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
I6J83_RS18405 (I6J83_18405)	aroE	3859385..3860203 (+)	819	WP_000451230.1	shikimate dehydrogenase	-
I6J83_RS18410 (I6J83_18410)	yrdB	3860200..3860457 (+)	258	WP_001070567.1	DUF1488 domain-containing protein	-
I6J83_RS18415 (I6J83_18415)	yrdA	3860433..3860987 (-)	555	WP_001286206.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40892.82 Da Isoelectric Point: 6.0395

                                >NTDB_id=535487 I6J83_RS18385 WP_000228508.1 3856663..3857787(+) (dprA) [Escherichia coli strain FDAARGOS_1253]

MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=535487 I6J83_RS18385 WP_000228508.1 3856663..3857787(+) (dprA) [Escherichia coli strain FDAARGOS_1253]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATTAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTATCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGCCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAGGGGGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (DprA_WH)

(312-357)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0D8VMA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	43.917	90.107	0.396
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0535487