NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	I6J85_RS03985	Genome accession	NZ_CP069447
Coordinates	836372..837496 (+)	Length	374 a.a.
NCBI ID	WP_000228508.1	Uniprot ID	A0A0D8VMA7
Organism	Escherichia coli strain FDAARGOS_1255
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 831372..842496

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6J85_RS03960 (I6J85_03960)	mscL	831489..831902 (-)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-
I6J85_RS03965 (I6J85_03965)	trkA	832032..833408 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
I6J85_RS03970 (I6J85_03970)	rsmB	833430..834719 (-)	1290	WP_000744773.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
I6J85_RS03975 (I6J85_03975)	fmt	834771..835718 (-)	948	WP_000004425.1	methionyl-tRNA formyltransferase	-
I6J85_RS03980 (I6J85_03980)	def	835733..836242 (-)	510	WP_000114984.1	peptide deformylase	-
I6J85_RS03985 (I6J85_03985)	dprA	836372..837496 (+)	1125	WP_000228508.1	DNA-protecting protein DprA	Machinery gene
I6J85_RS03990 (I6J85_03990)	smg	837468..837941 (+)	474	WP_000460672.1	DUF494 family protein Smg	-
I6J85_RS03995 (I6J85_03995)	yrdD	837970..838512 (+)	543	WP_001312137.1	type I DNA topoisomerase	-
I6J85_RS04000 (I6J85_04000)	tsaC	838517..839089 (+)	573	WP_001296460.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
I6J85_RS04005 (I6J85_04005)	aroE	839094..839912 (+)	819	WP_000451246.1	shikimate dehydrogenase	-
I6J85_RS04010 (I6J85_04010)	yrdB	839909..840166 (+)	258	WP_001070581.1	DUF1488 domain-containing protein	-
I6J85_RS04015 (I6J85_04015)	yrdA	840142..840696 (-)	555	WP_001286206.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40892.82 Da Isoelectric Point: 6.0395

                                >NTDB_id=534794 I6J85_RS03985 WP_000228508.1 836372..837496(+) (dprA) [Escherichia coli strain FDAARGOS_1255]

MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=534794 I6J85_RS03985 WP_000228508.1 836372..837496(+) (dprA) [Escherichia coli strain FDAARGOS_1255]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATTAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (DprA_WH)

(312-357)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0D8VMA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	43.917	90.107	0.396
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0534794