Detailed information NTG0534388

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: JR311_RS12955
• Genome accession: NZ_CP069391
• Coordinates: 2686533..2687147 (-)
• Length: 204 a.a.
• NCBI ID: WP_071391595.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain Sam8H1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2681533..2692147

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JR311_RS12925 (JR311_12925)	rpsT	2681833..2682099 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
JR311_RS12930 (JR311_12930)	holA	2682116..2683157 (-)	1042	Protein_2514	DNA polymerase III subunit delta	-
JR311_RS12935 (JR311_12935)	-	2683197..2683351 (-)	155	Protein_2515	hypothetical protein	-
JR311_RS12940 (JR311_12940)	-	2683392..2683526 (+)	135	WP_003152870.1	YqzM family protein	-
JR311_RS12945 (JR311_12945)	comEC	2683545..2685896 (-)	2352	WP_204529794.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
JR311_RS12950 (JR311_12950)	-	2685897..2686466 (-)	570	WP_003152868.1	ComE operon protein 2	-
JR311_RS12955 (JR311_12955)	comEA	2686533..2687147 (-)	615	WP_071391595.1	helix-hairpin-helix domain-containing protein	Machinery gene
JR311_RS12960 (JR311_12960)	comER	2687206..2688027 (+)	822	WP_015240246.1	late competence protein ComER	-
JR311_RS12965 (JR311_12965)	-	2688096..2688833 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
JR311_RS12970 (JR311_12970)	rsfS	2688830..2689186 (-)	357	WP_003152864.1	ribosome silencing factor	-
JR311_RS12975 (JR311_12975)	yqeK	2689204..2689764 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
JR311_RS12980 (JR311_12980)	-	2689754..2690323 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
JR311_RS12985 (JR311_12985)	yhbY	2690334..2690624 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
JR311_RS12990 (JR311_12990)	aroE	2690618..2691460 (-)	843	WP_165409541.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22218.33 Da
• Isoelectric Point: 5.4841

>NTDB_id=534388 JR311_RS12955 WP_071391595.1 2686533..2687147(-) (comEA) [Bacillus velezensis strain Sam8H1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTVSASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=534388 JR311_RS12955 WP_071391595.1 2686533..2687147(-) (comEA) [Bacillus velezensis strain Sam8H1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGTCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363