NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	JOF24_RS12375	Genome accession	NZ_CP069214
Coordinates	2561218..2561832 (-)	Length	204 a.a.
NCBI ID	WP_015240245.1	Uniprot ID	-
Organism	Bacillus velezensis strain GUAL210
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2556218..2566832

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JOF24_RS12345	rpsT	2556521..2556787 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
JOF24_RS12350	holA	2556804..2557845 (-)	1042	Protein_2407	DNA polymerase III subunit delta	-
JOF24_RS12355	-	2557885..2558036 (-)	152	Protein_2408	hypothetical protein	-
JOF24_RS12360	-	2558077..2558211 (+)	135	WP_003152870.1	YqzM family protein	-
JOF24_RS12365	comEC	2558230..2560581 (-)	2352	WP_061890729.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
JOF24_RS12370	-	2560582..2561151 (-)	570	WP_003152868.1	ComE operon protein 2	-
JOF24_RS12375	comEA	2561218..2561832 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
JOF24_RS12380	comER	2561891..2562712 (+)	822	WP_039254409.1	late competence protein ComER	-
JOF24_RS12385	-	2562781..2563518 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
JOF24_RS12390	rsfS	2563515..2563871 (-)	357	WP_007408260.1	ribosome silencing factor	-
JOF24_RS12395	yqeK	2563889..2564449 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
JOF24_RS12400	-	2564439..2565008 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
JOF24_RS12405	yhbY	2565019..2565309 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
JOF24_RS12410	aroE	2565303..2566145 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22209.32 Da Isoelectric Point: 5.5211

                                >NTDB_id=532980 JOF24_RS12375 WP_015240245.1 2561218..2561832(-) (comEA) [Bacillus velezensis strain GUAL210]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=532980 JOF24_RS12375 WP_015240245.1 2561218..2561832(-) (comEA) [Bacillus velezensis strain GUAL210]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0532980