Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LLNCDO700_RS07205 Genome accession   NZ_CP069179
Coordinates   1415211..1416503 (+) Length   430 a.a.
NCBI ID   WP_259691956.1    Uniprot ID   -
Organism   Lactococcus cremoris strain NCDO700     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1410211..1421503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLNCDO700_RS07180 (LLNCDO700_07175) glyQ 1410833..1411786 (-) 954 WP_011835327.1 glycine--tRNA ligase subunit alpha -
  LLNCDO700_RS07185 (LLNCDO700_07180) - 1412015..1412782 (-) 768 WP_011835328.1 5'-nucleotidase -
  LLNCDO700_RS07190 (LLNCDO700_07185) - 1412892..1413911 (-) 1020 WP_011835329.1 DUF475 domain-containing protein -
  LLNCDO700_RS07195 (LLNCDO700_07190) - 1413977..1414477 (-) 501 WP_259691955.1 VanZ family protein -
  LLNCDO700_RS07200 (LLNCDO700_07195) - 1414519..1415154 (-) 636 WP_011835331.1 YigZ family protein -
  LLNCDO700_RS07205 (LLNCDO700_07200) comFA 1415211..1416503 (+) 1293 WP_259691956.1 DEAD/DEAH box helicase Machinery gene
  LLNCDO700_RS07210 (LLNCDO700_07205) comFC 1416503..1417153 (+) 651 WP_259691957.1 ComF family protein Machinery gene
  LLNCDO700_RS07215 (LLNCDO700_07210) - 1417212..1417790 (-) 579 WP_011835334.1 nucleotidyltransferase family protein -
  LLNCDO700_RS07220 (LLNCDO700_07215) - 1417807..1418262 (-) 456 WP_011676094.1 diacylglycerol kinase family protein -
  LLNCDO700_RS07225 (LLNCDO700_07220) ybeY 1418246..1418734 (-) 489 WP_011835335.1 rRNA maturation RNase YbeY -
  LLNCDO700_RS07230 (LLNCDO700_07225) - 1418964..1419452 (-) 489 WP_259687581.1 NUDIX hydrolase -
  LLNCDO700_RS07235 (LLNCDO700_07230) - 1419522..1420490 (-) 969 WP_011835337.1 PhoH family protein -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 49451.61 Da        Isoelectric Point: 9.9193

>NTDB_id=532739 LLNCDO700_RS07205 WP_259691956.1 1415211..1416503(+) (comFA) [Lactococcus cremoris strain NCDO700]
MNLILENLLGRLLLEKDISAFYNFTDQVNNENKLIKICAMTSVSANKVRCNRCGTIHIKTNVKLPIGAFFCPTCLELGRV
RSDEYFYHLPQQDFPEKTYLRWTGKLTENQEKISNALCQQITNNQKLLVQAVTGAGKTEMIYQLIEQILSHGGSVGLASP
RIDVCIELHQRLSRDFTCQISLLYHEGDSYFRSPLVVMTSHQLLRFKEAFDLLIIDEVDAFPFRDNDMLYFALENAKKIN
GNLLYLTATSTDKLDKQIKKHEIKRLFLPRRFHGHPLVIPMFFWKKTFYKKFIEQRKTGFPLLIFVAEIDFGQDFAKNLQ
EKFQKERMAFVASTTKSRKTIVEAFRKKQVSILITTSILERGVTFSSIDVFVINSEHPNFTKSALIQMVGRVGRDSKRPT
GLVSFFHSGKSLAMCQAQKEIKKMNQLGGF

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=532739 LLNCDO700_RS07205 WP_259691956.1 1415211..1416503(+) (comFA) [Lactococcus cremoris strain NCDO700]
ATGAACTTAATTTTAGAAAATCTCCTTGGGCGTCTTTTACTAGAAAAAGATATTTCAGCTTTTTATAATTTTACTGACCA
AGTTAACAATGAAAATAAATTAATTAAAATTTGTGCAATGACATCTGTCAGTGCTAATAAAGTAAGATGTAATCGATGTG
GCACGATTCATATAAAAACCAATGTTAAACTTCCGATTGGTGCTTTTTTCTGTCCAACTTGTCTTGAACTTGGTCGTGTC
CGCTCAGATGAATATTTCTACCATCTTCCTCAACAAGACTTTCCAGAAAAAACTTATTTACGTTGGACTGGAAAATTAAC
AGAAAATCAAGAAAAAATTTCTAACGCTCTCTGTCAGCAAATTACTAATAATCAAAAACTACTTGTTCAAGCCGTTACTG
GTGCTGGTAAAACAGAAATGATATATCAGCTTATTGAACAAATTCTTAGCCATGGGGGTTCGGTCGGTTTAGCCAGCCCA
AGAATTGATGTATGCATTGAGCTTCATCAACGTTTATCACGTGATTTCACTTGTCAAATTTCCCTTTTGTATCATGAGGG
AGATTCTTATTTTCGTTCGCCTTTGGTCGTTATGACCAGTCACCAACTTTTACGTTTCAAAGAGGCCTTTGATTTGCTTA
TCATCGATGAAGTCGATGCCTTTCCTTTTAGAGATAATGACATGCTTTATTTCGCTCTTGAAAATGCTAAAAAAATTAAT
GGAAACTTGCTCTACTTAACCGCTACATCTACTGACAAATTAGACAAACAGATAAAAAAGCATGAAATTAAACGACTTTT
TTTACCACGCCGCTTTCACGGTCATCCTTTGGTCATTCCAATGTTTTTTTGGAAAAAAACATTTTATAAAAAATTCATTG
AGCAAAGAAAGACTGGTTTCCCACTTCTAATTTTCGTTGCCGAAATCGACTTTGGTCAAGACTTTGCCAAAAATTTACAA
GAAAAATTTCAAAAAGAAAGAATGGCTTTTGTCGCTTCAACAACTAAAAGCAGAAAAACGATTGTCGAAGCTTTTCGTAA
GAAACAAGTTTCGATTTTAATTACGACTTCTATTCTTGAACGAGGCGTAACTTTTTCTTCGATTGATGTTTTTGTCATTA
ATAGCGAACACCCTAATTTCACAAAATCTGCCCTCATTCAAATGGTCGGACGAGTGGGACGAGACTCTAAACGTCCAACA
GGATTAGTTAGTTTTTTTCACTCTGGAAAATCTTTAGCCATGTGTCAAGCTCAGAAAGAAATTAAAAAAATGAACCAACT
CGGAGGTTTTTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(105-249)

Helicase conserved C-terminal domain (Helicase_C)

(290-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

97.442

100

0.974
  comFA/cflA Streptococcus mitis SK321

49.657

100

0.505
  comFA/cflA Streptococcus pneumoniae D39

49.428

100

0.502
  comFA/cflA Streptococcus pneumoniae Rx1

49.428

100

0.502
  comFA/cflA Streptococcus pneumoniae R6

49.428

100

0.502
  comFA/cflA Streptococcus pneumoniae TIGR4

49.428

100

0.502
  comFA/cflA Streptococcus mitis NCTC 12261

48.97

100

0.498
  comFA Bacillus subtilis subsp. subtilis str. 168

37.225

100

0.393