Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   K9U07_RS10360 Genome accession   NZ_CP083512
Coordinates   2119326..2120819 (-) Length   497 a.a.
NCBI ID   WP_001600479.1    Uniprot ID   -
Organism   Escherichia coli strain elppa4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2114326..2125819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9U07_RS10330 (K9U07_10360) gspK 2115120..2116097 (-) 978 WP_000633199.1 type II secretion system minor pseudopilin GspK -
  K9U07_RS10335 (K9U07_10365) gspJ 2116094..2116699 (-) 606 WP_001600475.1 type II secretion system minor pseudopilin GspJ -
  K9U07_RS10340 (K9U07_10370) gspI 2116696..2117067 (-) 372 WP_000820112.1 type II secretion system minor pseudopilin GspI -
  K9U07_RS10345 (K9U07_10375) gspH 2117064..2117627 (-) 564 WP_001600477.1 type II secretion system minor pseudopilin GspH -
  K9U07_RS10350 (K9U07_10380) gspG 2117631..2118086 (-) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  K9U07_RS10355 (K9U07_10385) gspF 2118103..2119326 (-) 1224 WP_001600478.1 type II secretion system inner membrane protein GspF -
  K9U07_RS10360 (K9U07_10390) pilB/pilB1 2119326..2120819 (-) 1494 WP_001600479.1 type II secretion system ATPase GspE Machinery gene
  K9U07_RS10365 (K9U07_10395) gspD 2120819..2122879 (-) 2061 WP_228587446.1 type II secretion system secretin GspD -
  K9U07_RS10370 (K9U07_10400) gspC 2122909..2123868 (-) 960 WP_024190717.1 type II secretion system protein GspC -
  K9U07_RS10375 (K9U07_10405) gspS2 2123886..2124296 (-) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  K9U07_RS10380 (K9U07_10410) pilD 2124362..2125171 (-) 810 WP_001600480.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55252.44 Da        Isoelectric Point: 6.0390

>NTDB_id=532654 K9U07_RS10360 WP_001600479.1 2119326..2120819(-) (pilB/pilB1) [Escherichia coli strain elppa4]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCTDCRQALPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=532654 K9U07_RS10360 WP_001600479.1 2119326..2120819(-) (pilB/pilB1) [Escherichia coli strain elppa4]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTTTGGCAGCGTGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCCGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGCCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTACTGGTATCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGCCGGGCGATTGACGTGCGCGTCTCCACCATGCCATCCGCCTGGGGTG
AGCGCGTTGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACA
GTGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGACCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTCAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGGGTAGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCACCGACTGCCGCCAGGCCCTGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTCCGCGGGCGTACTGCCATCCATGAGTTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.741

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.633

80.684

0.376
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilF Thermus thermophilus HB27

45

80.483

0.362