Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   DI291_RS13890 Genome accession   NZ_CP068988
Coordinates   2769921..2770550 (-) Length   209 a.a.
NCBI ID   WP_096748238.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain SUBG0010     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2764921..2775550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DI291_RS13870 (DI291_14285) holA 2765480..2766505 (-) 1026 WP_188016690.1 DNA polymerase III subunit delta -
  DI291_RS13875 (DI291_14290) - 2766735..2766866 (+) 132 WP_003183686.1 YqzM family protein -
  DI291_RS13880 (DI291_14295) - 2766891..2769278 (-) 2388 WP_205802133.1 DNA internalization-related competence protein ComEC/Rec2 -
  DI291_RS13885 (DI291_14300) - 2769283..2769852 (-) 570 WP_020452273.1 ComE operon protein 2 -
  DI291_RS13890 (DI291_14305) comEA 2769921..2770550 (-) 630 WP_096748238.1 helix-hairpin-helix domain-containing protein Machinery gene
  DI291_RS13895 (DI291_14310) comER 2770633..2771454 (+) 822 WP_229128976.1 late competence protein ComER -
  DI291_RS13900 (DI291_14315) - 2771525..2772280 (-) 756 WP_023855255.1 class I SAM-dependent methyltransferase -
  DI291_RS13905 (DI291_14320) rsfS 2772277..2772633 (-) 357 WP_023855256.1 ribosome silencing factor -
  DI291_RS13910 (DI291_14325) yqeK 2772647..2773210 (-) 564 WP_026579268.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  DI291_RS13915 (DI291_14330) - 2773200..2773769 (-) 570 WP_025811125.1 nicotinate-nucleotide adenylyltransferase -
  DI291_RS13920 (DI291_14335) yhbY 2773788..2774078 (-) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  DI291_RS13925 (DI291_14340) aroE 2774072..2774908 (-) 837 WP_096748242.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22628.43 Da        Isoelectric Point: 4.6492

>NTDB_id=531855 DI291_RS13890 WP_096748238.1 2769921..2770550(-) (comEA) [Bacillus paralicheniformis strain SUBG0010]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGFSIPEEASAQTFDKKEEVKREKNAGEEEVIIDLKGAVKNPGV
YQMKEGDRVHDALKKAGGTEKKADQKQINLAAVLRDGMVLYIPFEGEEAAGSLSEADSRADGSSSDIVNINTASSEELQT
IPGIGPSKAEAVVEYREENGMFQTIEDITNVSGIGEKSFERIKSSITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=531855 DI291_RS13890 WP_096748238.1 2769921..2770550(-) (comEA) [Bacillus paralicheniformis strain SUBG0010]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCGGCGGGAGGTGTGGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCTTCTCCATTCCTGAAGAGGCTTCTGCACAGACTTTTGACAAGAAAG
AGGAGGTGAAGCGGGAAAAAAACGCGGGGGAAGAGGAGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAGGAGGGAGACAGGGTGCACGATGCATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGACCAAAAGCA
GATTAATCTGGCAGCCGTTTTGCGGGACGGTATGGTTCTTTACATTCCATTTGAAGGGGAAGAGGCCGCTGGCTCTCTTT
CGGAAGCGGACTCAAGAGCAGACGGCAGCTCAAGTGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGACG
ATTCCCGGGATCGGTCCTTCAAAAGCGGAAGCGGTAGTCGAATACCGCGAGGAGAACGGAATGTTTCAGACGATTGAAGA
CATAACAAACGTTTCTGGAATTGGAGAGAAGTCTTTTGAAAGAATAAAATCTTCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(69-122)

Helix-hairpin-helix motif (HHH_3)

(145-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

52.336

100

0.536
  comEA Staphylococcus aureus MW2

36.364

100

0.383
  comEA Staphylococcus aureus N315

35.909

100

0.378
  comEA Lactococcus lactis subsp. cremoris KW2

35.023

100

0.364