Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   JN403_RS02300 Genome accession   NZ_CP068986
Coordinates   532529..533593 (-) Length   354 a.a.
NCBI ID   WP_074890782.1    Uniprot ID   A0A1I0G993
Organism   Pseudomonas sp. 15A4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 527529..538593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JN403_RS02275 (JN403_02275) aroK 528135..528653 (-) 519 WP_065986620.1 shikimate kinase AroK -
  JN403_RS02280 (JN403_02280) pilQ 528658..530740 (-) 2083 Protein_442 type IV pilus secretin PilQ -
  JN403_RS02285 (JN403_02285) - 530794..531320 (-) 527 Protein_443 pilus assembly protein PilP -
  JN403_RS02290 (JN403_02290) pilO 531317..531964 (-) 648 WP_122533930.1 type 4a pilus biogenesis protein PilO -
  JN403_RS02295 (JN403_02295) - 531951..532529 (-) 579 WP_205485017.1 PilN domain-containing protein -
  JN403_RS02300 (JN403_02300) pilM 532529..533593 (-) 1065 WP_074890782.1 pilus assembly protein PilM Machinery gene
  JN403_RS02305 (JN403_02305) - 533799..536276 (+) 2478 WP_205485019.1 penicillin-binding protein 1A -
  JN403_RS02310 (JN403_02310) - 536412..537680 (-) 1269 WP_122533933.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38219.81 Da        Isoelectric Point: 4.6363

>NTDB_id=531794 JN403_RS02300 WP_074890782.1 532529..533593(-) (pilM) [Pseudomonas sp. 15A4]
MFELFSKKANTLLGIDISSTSVKLLELSRSGNRYKVESYAVEPLPANAVVEKNIAELEGVGSALTRLLVKAKSNVKIAAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLSAQLGQDHEQLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSV
SEAGLAKKQGGLPDDYVMEVLQPFKDAVVQQVSRSLQFFFAAGQYNRVDYIMLAGGTASISGLDRLIQERLGTPTLVANP
FADMALSVKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=531794 JN403_RS02300 WP_074890782.1 532529..533593(-) (pilM) [Pseudomonas sp. 15A4]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTGGGGATCGATATTAGCTCCACCTCGGTCAAGCTCCTGGAGCT
AAGTCGTTCCGGTAACCGTTACAAGGTCGAGTCTTATGCGGTCGAGCCACTGCCCGCCAACGCAGTGGTCGAAAAGAACA
TCGCGGAGCTCGAAGGTGTCGGCAGTGCTTTGACGCGGCTGCTGGTAAAAGCCAAAAGTAACGTCAAAATCGCGGCGGTT
GCCGTCGCCGGTTCGGCTGTTATTACCAAGACCATCGAGATGGACGCAGGCCTTTCCGACGATGAGATGGAAAACCAGCT
CAAGATCGAGGCCGATCAGTACATTCCCTATCCGCTTGAAGAAGTCGCCATCGACTTCGAAGTCCAGGGCTATTCGGCGC
GCAATCCCGAACGCGTTGAGGTATTGCTCGCCGCCTGCCGCAAGGAAAACGTAGAAGTTCGCGAAGCCGCGCTGGCACTG
GCCGGGCTCACCGCTCGCGTGGTCGACGTCGAAGCCTATGCCTTGGAGCGCTCGTTCGGTCTGCTGTCGGCGCAATTGGG
CCAGGACCACGAACAGTTGACCGTCGCCGTCATAGACATCGGCGCAACCATGACCACCCTCAGCGTGCTGCACAACGGCC
GCATCATCTATACCCGCGAACAATTATTTGGCGGTCGCCAGTTAACCGAAGAAATCCAGCGCCGTTATGGCTTGTCGGTC
AGCGAGGCCGGGCTTGCGAAAAAGCAGGGCGGACTCCCCGACGACTACGTTATGGAAGTCCTGCAGCCGTTCAAGGACGC
CGTGGTGCAACAGGTGTCTCGTTCTCTGCAGTTTTTCTTCGCGGCCGGCCAGTACAACCGCGTCGACTACATCATGCTGG
CCGGCGGCACCGCCTCTATTTCCGGTCTTGATCGGCTGATCCAGGAACGCCTGGGCACGCCGACGCTGGTGGCCAACCCC
TTTGCTGACATGGCGCTGAGCGTCAAGGTCAACGCCGGCGCCCTCGCCAGCGATGCTCCCGCATTGATGATCGCTTGCGG
TCTGGCCCTGAGGAGCTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I0G993

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466