Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   JHS79_RS15740 Genome accession   NZ_CP068641
Coordinates   3162476..3163069 (-) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain VP157     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3157476..3168069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHS79_RS15720 (JHS79_15570) aroB 3158352..3159452 (-) 1101 WP_005480443.1 3-dehydroquinate synthase -
  JHS79_RS15725 (JHS79_15575) aroK 3159484..3160002 (-) 519 WP_005381319.1 shikimate kinase AroK -
  JHS79_RS15730 (JHS79_15580) pilQ 3160191..3161939 (-) 1749 WP_025556542.1 type IV pilus secretin PilQ Machinery gene
  JHS79_RS15735 (JHS79_15585) pilP 3161971..3162486 (-) 516 WP_025525190.1 pilus assembly protein PilP Machinery gene
  JHS79_RS15740 (JHS79_15590) pilO 3162476..3163069 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  JHS79_RS15745 (JHS79_15595) pilN 3163062..3163640 (-) 579 WP_075111203.1 PilN domain-containing protein Machinery gene
  JHS79_RS15750 (JHS79_15600) pilM 3163624..3164640 (-) 1017 WP_075111204.1 type IV pilus biogenesis protein PilM Machinery gene
  JHS79_RS15755 (JHS79_15605) - 3164783..3167353 (+) 2571 WP_140126307.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=530179 JHS79_RS15740 WP_005480482.1 3162476..3163069(-) (pilO) [Vibrio parahaemolyticus strain VP157]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=530179 JHS79_RS15740 WP_005480482.1 3162476..3163069(-) (pilO) [Vibrio parahaemolyticus strain VP157]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACATTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726