Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   JJQ59_RS02925 Genome accession   NZ_CP068434
Coordinates   601812..602324 (+) Length   170 a.a.
NCBI ID   WP_114133954.1    Uniprot ID   A0A367PF16
Organism   Cupriavidus necator strain C39.     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 596812..607324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJQ59_RS02900 (JJQ59_02900) recX 596852..597319 (+) 468 WP_114133956.1 recombination regulator RecX -
  JJQ59_RS02905 (JJQ59_02905) - 597629..598324 (+) 696 WP_114133955.1 DUF2889 domain-containing protein -
  JJQ59_RS02910 (JJQ59_02910) sucC 598414..599580 (+) 1167 WP_013955623.1 ADP-forming succinate--CoA ligase subunit beta -
  JJQ59_RS02915 (JJQ59_02915) sucD 599680..600561 (+) 882 WP_011614568.1 succinate--CoA ligase subunit alpha -
  JJQ59_RS02920 (JJQ59_02920) - 600799..601494 (+) 696 WP_013955624.1 TerC family protein -
  JJQ59_RS02925 (JJQ59_02925) pilA2 601812..602324 (+) 513 WP_114133954.1 pilin Machinery gene
  JJQ59_RS02930 (JJQ59_02930) - 602419..604221 (+) 1803 WP_114133953.1 PglL family O-oligosaccharyltransferase -
  JJQ59_RS02935 (JJQ59_02935) - 604223..604741 (-) 519 WP_114133963.1 hypothetical protein -
  JJQ59_RS02940 (JJQ59_02940) - 604756..605190 (-) 435 WP_011614573.1 hypothetical protein -
  JJQ59_RS02945 (JJQ59_02945) moaC 605419..605898 (-) 480 WP_114133952.1 cyclic pyranopterin monophosphate synthase MoaC -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 16966.65 Da        Isoelectric Point: 10.0092

>NTDB_id=529042 JJQ59_RS02925 WP_114133954.1 601812..602324(+) (pilA2) [Cupriavidus necator strain C39.]
MQRVQLKKLGRRVQKGFTLIELMIVVAIIGILAAIAIPAYQDYVIRARVTEGLSLADTAKTSITENAANGVALANGWVAP
AATQNVTGIAVAANGQITITYAARAGNGTLVLVPSSGAAALVAGTPPAGGSVDWECFSAAKAASTLPAPGPAPAAAATLA
GNFAPVSCRT

Nucleotide


Download         Length: 513 bp        

>NTDB_id=529042 JJQ59_RS02925 WP_114133954.1 601812..602324(+) (pilA2) [Cupriavidus necator strain C39.]
ATGCAACGGGTACAACTGAAGAAACTGGGCCGTCGTGTCCAGAAGGGCTTTACGCTGATCGAACTGATGATCGTGGTTGC
GATCATTGGTATTTTGGCGGCGATCGCGATTCCGGCGTATCAAGACTACGTGATTCGCGCCCGTGTTACGGAAGGCTTGT
CATTGGCTGATACGGCGAAGACTTCCATCACCGAGAACGCAGCTAACGGCGTTGCCCTGGCAAACGGCTGGGTAGCTCCT
GCTGCTACTCAAAACGTTACCGGTATCGCCGTTGCCGCTAATGGACAAATCACCATCACCTACGCCGCTCGTGCGGGCAA
TGGTACGCTCGTACTCGTTCCGTCGTCCGGCGCCGCTGCATTGGTTGCCGGTACTCCGCCGGCTGGTGGTTCGGTGGATT
GGGAATGCTTCTCGGCAGCCAAGGCCGCTTCGACTCTGCCGGCTCCTGGCCCGGCGCCGGCCGCTGCAGCAACTCTGGCG
GGCAACTTTGCGCCGGTAAGCTGCCGCACCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-36)

Pilin (bacterial filament) (Pilin)

(46-169)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A367PF16

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

54.037

94.706

0.512
  pilA Ralstonia pseudosolanacearum GMI1000

51.479

99.412

0.512
  pilA2 Legionella pneumophila strain ERS1305867

53.416

94.706

0.506
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.233

100

0.4