Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   K8B89_RS21100 Genome accession   NZ_CP082775
Coordinates   4266741..4267400 (+) Length   219 a.a.
NCBI ID   WP_001221490.1    Uniprot ID   -
Organism   Escherichia coli O25:H4 strain CU568-3     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4261741..4272400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B89_RS21085 (K8B89_20945) ygiS 4263946..4265553 (-) 1608 WP_000288656.1 ABC transporter substrate-binding protein -
  K8B89_RS21090 (K8B89_20950) ygiV 4265662..4266144 (-) 483 WP_000183484.1 GyrI-like domain-containing protein -
  K8B89_RS21095 (K8B89_20955) ygiW 4266197..4266589 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  K8B89_RS21100 (K8B89_20960) ciaR 4266741..4267400 (+) 660 WP_001221490.1 quorum sensing response regulator transcription factor QseB Regulator
  K8B89_RS21105 (K8B89_20965) qseC 4267397..4268746 (+) 1350 WP_000673389.1 quorum sensing histidine kinase QseC -
  K8B89_RS21110 (K8B89_20970) ygiZ 4268792..4269124 (-) 333 WP_000914696.1 DUF2645 family protein -
  K8B89_RS21115 - 4269131..4269841 (-) 711 WP_000834022.1 hypothetical protein -
  K8B89_RS21120 - 4269844..4270323 (-) 480 WP_000065331.1 Hcp family type VI secretion system effector -
  K8B89_RS21125 (K8B89_20980) mdaB 4270573..4271154 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  K8B89_RS21130 (K8B89_20985) ygiN 4271185..4271499 (+) 315 WP_000633738.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24663.65 Da        Isoelectric Point: 6.8808

>NTDB_id=528894 K8B89_RS21100 WP_001221490.1 4266741..4267400(+) (ciaR) [Escherichia coli O25:H4 strain CU568-3]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELLRGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=528894 K8B89_RS21100 WP_001221490.1 4266741..4267400(+) (ciaR) [Escherichia coli O25:H4 strain CU568-3]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCTCCGCGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365
  covR Lactococcus lactis subsp. lactis strain DGCC12653

34.802

100

0.361