Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   E1N14_RS02185 Genome accession   NZ_CP068230
Coordinates   458000..458914 (-) Length   304 a.a.
NCBI ID   WP_062793712.1    Uniprot ID   -
Organism   Shewanella algae strain CECT-5071     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453000..463914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E1N14_RS02165 (E1N14_002165) mutT 455996..456394 (-) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -
  E1N14_RS02170 (E1N14_002170) yacG 456394..456606 (-) 213 WP_025012057.1 DNA gyrase inhibitor YacG -
  E1N14_RS02175 (E1N14_002175) zapD 456651..457391 (-) 741 WP_028779562.1 cell division protein ZapD -
  E1N14_RS02180 (E1N14_002180) coaE 457388..457999 (-) 612 WP_025012056.1 dephospho-CoA kinase -
  E1N14_RS02185 (E1N14_002185) pilD 458000..458914 (-) 915 WP_062793712.1 prepilin peptidase Machinery gene
  E1N14_RS02190 (E1N14_002190) pilC 458983..460248 (-) 1266 WP_025012055.1 type II secretion system F family protein Machinery gene
  E1N14_RS02195 (E1N14_002195) pilB 460401..462107 (-) 1707 WP_025012054.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E1N14_RS02200 (E1N14_002200) - 462117..462530 (-) 414 WP_025012053.1 pilin -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 33869.24 Da        Isoelectric Point: 7.2147

>NTDB_id=528307 E1N14_RS02185 WP_062793712.1 458000..458914(-) (pilD) [Shewanella algae strain CECT-5071]
MSEFIAVLSHNQWLFLVLCFLFASVIGSFLNVVIHRLPVMMKREWQQECNQYLQEYQPEFAKQLGEAKLNAPIDNYPAKY
NLLLPKSACPKCGAHIKPWHNLPIIGYLLLGGKCASCNTAISARYPIFELLTALLISTLGWYFGPTLQFVYATILTFGLI
ALSGIDLDEMLLPDQITLPLLWLGLLVNISGTFTNLTDATIGATAGYLSLWSVYWLFKLLTGKEGMGYGDFKLMALFGAW
LGWQMLPLIILLSSLVGALVGVLLILFKNLNRGNPIPFGPYIAIAGWVALVWGQQIVDWYLGTL

Nucleotide


Download         Length: 915 bp        

>NTDB_id=528307 E1N14_RS02185 WP_062793712.1 458000..458914(-) (pilD) [Shewanella algae strain CECT-5071]
ATGTCAGAATTTATTGCCGTATTGAGCCACAACCAGTGGCTCTTTCTTGTTCTCTGTTTTTTGTTTGCCTCTGTAATAGG
CAGCTTTCTCAATGTGGTAATCCATCGCCTGCCGGTAATGATGAAGCGCGAATGGCAGCAGGAATGCAATCAATACCTGC
AGGAATATCAACCCGAATTCGCCAAACAGCTGGGTGAAGCCAAGCTTAATGCACCAATAGATAACTATCCGGCCAAATAC
AATCTGTTACTGCCGAAATCTGCCTGCCCCAAGTGTGGCGCCCATATCAAACCCTGGCACAACCTCCCCATAATCGGCTA
TTTGTTGCTTGGCGGTAAATGCGCCTCCTGCAACACAGCTATTTCAGCGAGATACCCGATATTTGAGCTGCTGACCGCCC
TGCTTATCAGCACTCTCGGTTGGTATTTTGGCCCGACACTGCAGTTTGTTTACGCCACTATTCTCACCTTTGGATTGATA
GCCCTCAGCGGGATAGATCTGGATGAAATGCTGCTGCCGGATCAGATTACCCTGCCGCTACTGTGGCTTGGTTTACTGGT
TAATATCTCGGGCACCTTTACTAATCTGACCGATGCCACCATAGGGGCCACTGCCGGCTATTTAAGCCTCTGGTCGGTCT
ACTGGCTATTTAAACTGCTCACCGGCAAGGAAGGCATGGGCTATGGCGATTTCAAGCTGATGGCATTGTTTGGCGCCTGG
CTTGGCTGGCAGATGTTACCTCTAATTATTCTGCTCTCTTCTCTGGTCGGTGCCCTAGTAGGCGTTTTACTTATTCTGTT
CAAGAACCTTAATCGGGGTAACCCTATCCCCTTTGGCCCTTATATTGCTATCGCAGGTTGGGTCGCTTTGGTGTGGGGGC
AACAGATTGTCGACTGGTATTTGGGAACACTCTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-143)

Type IV leader peptidase family (Peptidase_A24)

(154-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

49.169

99.013

0.487
  pilD Vibrio cholerae strain A1552

50

96.053

0.48
  pilD Acinetobacter nosocomialis M2

48.013

99.342

0.477
  pilD Acinetobacter baumannii D1279779

47.682

99.342

0.474
  pilD Neisseria gonorrhoeae MS11

45.196

92.434

0.418