Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E1N14_RS02195 Genome accession   NZ_CP068230
Coordinates   460401..462107 (-) Length   568 a.a.
NCBI ID   WP_025012054.1    Uniprot ID   -
Organism   Shewanella algae strain CECT-5071     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 455401..467107
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E1N14_RS02165 (E1N14_002165) mutT 455996..456394 (-) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -
  E1N14_RS02170 (E1N14_002170) yacG 456394..456606 (-) 213 WP_025012057.1 DNA gyrase inhibitor YacG -
  E1N14_RS02175 (E1N14_002175) zapD 456651..457391 (-) 741 WP_028779562.1 cell division protein ZapD -
  E1N14_RS02180 (E1N14_002180) coaE 457388..457999 (-) 612 WP_025012056.1 dephospho-CoA kinase -
  E1N14_RS02185 (E1N14_002185) pilD 458000..458914 (-) 915 WP_062793712.1 prepilin peptidase Machinery gene
  E1N14_RS02190 (E1N14_002190) pilC 458983..460248 (-) 1266 WP_025012055.1 type II secretion system F family protein Machinery gene
  E1N14_RS02195 (E1N14_002195) pilB 460401..462107 (-) 1707 WP_025012054.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E1N14_RS02200 (E1N14_002200) - 462117..462530 (-) 414 WP_025012053.1 pilin -
  E1N14_RS02205 (E1N14_002205) nadC 463398..464258 (-) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E1N14_RS02210 (E1N14_002210) ampD 464417..464995 (+) 579 WP_051547208.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E1N14_RS02215 (E1N14_002215) ampE 465049..465900 (+) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  E1N14_RS02220 (E1N14_002220) pdhR 466175..466927 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63105.23 Da        Isoelectric Point: 5.3439

>NTDB_id=528309 E1N14_RS02195 WP_025012054.1 460401..462107(-) (pilB) [Shewanella algae strain CECT-5071]
MPTSGLHLGLSTLFIRKGLLTENQLTETIRHSQKNKQTLVTTLVASNLLSARTIAELCYEEYGTPLLDLNEFDLNSIPEE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLDLDGLDEES
LAGIEVTDTDKRQQEDGESKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMRLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPEHELLQLGFT
QQQIDAGFTPYKPCGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAATAKQQGMRDLRLSGLLKVIQGVTSIA
EINRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=528309 E1N14_RS02195 WP_025012054.1 460401..462107(-) (pilB) [Shewanella algae strain CECT-5071]
ATGCCAACATCCGGCTTACACCTAGGTCTGTCGACTCTTTTTATCCGTAAAGGTCTACTGACGGAAAATCAGTTAACCGA
AACGATAAGACATTCCCAGAAAAATAAACAAACACTGGTCACCACTCTGGTGGCCAGTAATCTTTTATCCGCTCGCACTA
TCGCCGAACTCTGCTATGAAGAATACGGCACCCCGCTGCTGGATCTCAATGAGTTTGATTTAAACAGTATCCCGGAAGAA
TTTCTCAATAAAAAGCTGATCGATAAGCACCGCTGCCTGCCGTTATTCAAGCGTGGCAACCGCCTCTATATCGCCACTTC
AGACCCCACCAATATTGCCGCCCTGGAAGATTTTCAGTTCAGCGCCGGCTTGCACGCCGAAGCGATACTGGTAGAGGAAG
ATAAGCTCAGTAAAGCACTGGAAAAAGTGCTTGAAGAGGACTTGAGTTCGCTGGATCTCGATGGCCTCGATGAGGAATCC
CTCGCAGGTATCGAGGTCACCGACACCGACAAACGTCAACAGGAAGATGGTGAAAGTAAAGATGACGCCCCCATCGTCAT
CTATATCAATAAGATTTTGACCGATGCCATCCGCAAGGGCGCATCGGATCTGCACTTCGAACCCTACGAGAAGCGCTACC
GCATCCGTTTCCGTATCGATGGCATCCTCCACGAGGTATCTGAGCCCCCCATTTCTCTCTCGGGCCGTATCTCAGCCCGC
TTGAAGGTGATGTCCAAATTGGATATCGCCGAGCGCCGCGTTCCTCAGGATGGCCGCATCAAGATGAGGCTGTCGCGCAA
CAAGAGTATCGATTTCCGGGTCAGTACCCTGCCCACTCTTTGGGGTGAAAAGATAGTGATGCGGATCCTCGACTCGTCTT
CCGCGCAATTAGGCATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTACCTGGAGATGCTGGCAAGGCCCCAG
GGGATGATTCTGGTGACAGGCCCTACCGGCTCTGGTAAAACCGTTTCCCTTTATACTGGCCTCAATATCCTCAATACCGA
AGAGCGCAATATCAGCACCGCCGAAGACCCAGTGGAGATCAACCTGGAAGGGGTCAACCAGGTACATATCAACCCCAAGG
CAGGGCTAACCTTCGCCTCGGCGCTGCGCTCCTTTTTGCGTCAGGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAC
CTGGAAACCGCCGAAATCGCCATTAAGGCGGCACAAACCGGCCACTTGGTACTCTCGACCCTGCATACCAACTCATCGGC
CGAGACTCTCACCCGCCTGCTTAATATGGGTGTTCCCGGCTATAATATCGCCAGCTCTGTCAACCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTCTGCCCCGAATGCCGCCAACCGGAAGATATTCCCGAGCATGAACTGCTGCAACTGGGCTTCACT
CAGCAGCAGATAGACGCAGGCTTTACCCCTTACAAGCCCTGTGGCTGCGATCTCTGCTCCGGTGGTTATAAGGGCCGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGACGAAATCGCCAGAACCATTATGGAGGGCGGTAATTCGCTGCAGATTGCCG
CCACTGCCAAACAGCAGGGGATGCGTGATTTGCGCCTTTCCGGTCTTTTAAAAGTGATTCAAGGCGTCACCAGTATCGCC
GAGATAAATCGGGTCACCAGCTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.537

98.768

0.588
  pilB Acinetobacter baumannii D1279779

60.223

94.718

0.57
  pilB Legionella pneumophila strain ERS1305867

55.83

99.648

0.556
  pilB Vibrio parahaemolyticus RIMD 2210633

52.91

99.824

0.528
  pilB Vibrio cholerae strain A1552

53.455

96.831

0.518
  pilB Vibrio campbellii strain DS40M4

51.332

99.12

0.509
  pilF Neisseria gonorrhoeae MS11

47.965

99.472

0.477
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.517

97.359

0.375
  pilF Thermus thermophilus HB27

37.411

99.296

0.371