Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JHW41_RS19050 Genome accession   NZ_CP067396
Coordinates   4602312..4603478 (-) Length   388 a.a.
NCBI ID   WP_057946566.1    Uniprot ID   A0A0S2DDC7
Organism   Lysobacter enzymogenes strain CX06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4590779..4602125 4602312..4603478 flank 187


Gene organization within MGE regions


Location: 4590779..4603478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW41_RS18995 (JHW41_18905) - 4590779..4591342 (-) 564 WP_250444702.1 hypothetical protein -
  JHW41_RS19000 (JHW41_18910) - 4591460..4592395 (-) 936 WP_250444704.1 aspartate carbamoyltransferase catalytic subunit -
  JHW41_RS19005 (JHW41_18915) ruvX 4592472..4592960 (-) 489 WP_057946573.1 Holliday junction resolvase RuvX -
  JHW41_RS19010 (JHW41_18920) - 4592957..4593523 (-) 567 WP_057946572.1 YqgE/AlgH family protein -
  JHW41_RS19015 (JHW41_18925) - 4593704..4594243 (+) 540 Protein_3738 DNA-3-methyladenine glycosylase I -
  JHW41_RS19020 (JHW41_18930) - 4594874..4596697 (+) 1824 WP_250444706.1 DUF4153 domain-containing protein -
  JHW41_RS19025 (JHW41_18935) - 4596864..4597937 (-) 1074 WP_250444708.1 Rieske 2Fe-2S domain-containing protein -
  JHW41_RS19030 (JHW41_18940) - 4597934..4599049 (-) 1116 WP_250444710.1 hypothetical protein -
  JHW41_RS19035 (JHW41_18945) - 4599046..4600206 (-) 1161 WP_250444712.1 ATP-grasp domain-containing protein -
  JHW41_RS19040 (JHW41_18950) - 4600613..4601518 (+) 906 WP_250444714.1 hypothetical protein -
  JHW41_RS19045 (JHW41_18955) - 4601643..4602125 (-) 483 WP_250444716.1 hypothetical protein -
  JHW41_RS19050 (JHW41_18960) pilU 4602312..4603478 (-) 1167 WP_057946566.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43065.41 Da        Isoelectric Point: 6.8286

>NTDB_id=525874 JHW41_RS19050 WP_057946566.1 4602312..4603478(-) (pilU) [Lysobacter enzymogenes strain CX06]
MNTPAPSNPTTASGAIDFTSFLKLMAHQRASDLFITAGMPPSMKVHGKITPITQNPLTPQQSRDLVLNVMSPQQREEFEK
THECNFAIGVTGVGRFRVSCFYQRNQVGMVLRRIETKIPSVDELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIG
YRNQNSTGHIITIEDPIEFVHRHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVL
CTLHANNANQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLAQDYIRDGEIHKLKEL
MKESTQLGMKTFDQALFELYQGGEISYEDALRYADSQNEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=525874 JHW41_RS19050 WP_057946566.1 4602312..4603478(-) (pilU) [Lysobacter enzymogenes strain CX06]
ATGAACACGCCCGCCCCGTCCAACCCGACCACCGCCTCCGGCGCGATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCA
CCAGCGCGCCTCGGACTTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTGCACGGCAAGATCACCCCGATCACCC
AGAACCCGCTGACCCCGCAGCAGAGCCGCGACCTCGTGCTCAACGTGATGAGCCCGCAGCAGCGCGAGGAGTTCGAAAAG
ACCCACGAGTGCAACTTCGCCATCGGCGTCACCGGTGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAATCAGGT
CGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCCCAGCGTCGACGAACTGAACCTGCCGCCGATCATCAAGACGCTGG
CGATGACCAAGCGCGGCATCATCATCTTCGTCGGCGCCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGC
TACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAATTCGTGCATCGGCACGAGGGCTGCAT
CATCACCCAGCGCGAAGTCGGCATCGACACCGACAGCTGGGAAGCCGCGCTCAAGAACACCCTGCGCCAGGCGCCGGACG
TGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTG
TGCACCCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAACCAGCT
GCTGATGGATCTGTCGCTGAACCTCAAGGGCGTGGTCGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGGGCCGGCGCG
TGGCGATGGAGATCCTGCTCGGCACCCCGCTGGCGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGCTG
ATGAAGGAATCCACCCAGTTGGGCATGAAGACCTTCGACCAGGCCCTGTTCGAGCTCTACCAGGGCGGCGAGATCAGCTA
CGAGGACGCGCTGCGCTACGCCGACTCGCAGAACGAAGTGCGCCTGCGGATCAAGCTGGCGCAGGGCGGCGACGCGCGGA
CCTTGTCGCAGGGGTTGGATGGGGTTGAGGTGGCGGAGGTGCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S2DDC7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.21

94.588

0.655
  pilU Acinetobacter baylyi ADP1

65.546

92.01

0.603
  pilU Vibrio cholerae strain A1552

53.09

91.753

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.595

90.464

0.376