Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JHT90_RS03990 Genome accession   NZ_CP067393
Coordinates   881302..883500 (+) Length   732 a.a.
NCBI ID   WP_201095757.1    Uniprot ID   A0A974NII7
Organism   Entomomonas asaccharolytica strain F2A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 876302..888500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHT90_RS03970 (JHT90_03970) pilM 878495..879553 (+) 1059 WP_379971852.1 type IV pilus assembly protein PilM -
  JHT90_RS03975 (JHT90_03975) - 879553..880122 (+) 570 WP_201094411.1 PilN domain-containing protein -
  JHT90_RS03980 (JHT90_03980) - 880119..880721 (+) 603 WP_201094413.1 type 4a pilus biogenesis protein PilO -
  JHT90_RS03985 (JHT90_03985) - 880718..881245 (+) 528 WP_236254034.1 pilus assembly protein PilP -
  JHT90_RS03990 (JHT90_03990) pilQ 881302..883500 (+) 2199 WP_201095757.1 type IV pilus secretin PilQ Machinery gene
  JHT90_RS03995 (JHT90_03995) aroK 883535..884020 (+) 486 WP_201094421.1 shikimate kinase AroK -
  JHT90_RS04000 (JHT90_04000) aroB 884065..885168 (+) 1104 WP_201094424.1 3-dehydroquinate synthase -
  JHT90_RS04005 (JHT90_04005) - 885173..886663 (+) 1491 WP_201094430.1 ATPase, T2SS/T4P/T4SS family -
  JHT90_RS04010 (JHT90_04010) queF 886666..887499 (+) 834 WP_201094438.1 NADPH-dependent 7-cyano-7-deazaguanine reductase QueF -
  JHT90_RS04015 (JHT90_04015) - 887530..887796 (-) 267 WP_201094439.1 DUF4404 family protein -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 79742.58 Da        Isoelectric Point: 5.5841

>NTDB_id=525816 JHT90_RS03990 WP_201095757.1 881302..883500(+) (pilQ) [Entomomonas asaccharolytica strain F2A]
MNVSLMRLSIALLSMAIPSMLYAANLKDMDVVSLPGERVELKLVFDEPVTAPKGYTIDQPARIALDLPGVSSLLGEKRRT
VGIGNVRNLTVVEAKNVTRLVVGLNTLVPYTTRAEGNNIFIVLGDSVSTSPIVPTNTVSTLPTKSTNTVTPVTTKSVASI
GNAIENIDFRRGDNGEGNIVVKLSNSTISPDVQERGGKIVLNFPKTQLPESLRVRLDVKDFATPVQFINSTAKSDGSSIT
IEPKGNYDYLVFQANDELTVSVKHVSVEEKQRRLEDQGIFKGDTLSLNFQDIEVRSVLQILADFTKLNLVATDSVRGNIT
LRLQNVPWDQALDIIMRTRGLAKRQEGNILIIAPADELAESARRMFDAEQTLKATQPLRREIIPVNYADATSIIALFQAT
MSQTNSIQAGQNASGMGNYSANMTTNDRGSLTVDKRTNTIIAYQTEERLEELRRIIAQLDRPVRQVQIEARIVEANVDFA
KNLGVRWGADFGSKSGNWQSYGGADGRKNSTGSSTEFGNLGTMFTNDGDGYIRTLAAPVVDLGAADATSGFGIGFLSSNV
MIDLKLNAMQRSGQGEVISQPKVTTSDKETARIMKGQEVPYQEASSSGATSTSFKEALLSLEVTPQITPDNQIMMAVVVT
KDSVNESQRYQGVPPINKNEVNAKVLVSDGETIVLGGVFQNDQSKITEKVPFLGDLPVMGRLFRRDSVSNTKQELLIFIT
PRIIDNKAFASR

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=525816 JHT90_RS03990 WP_201095757.1 881302..883500(+) (pilQ) [Entomomonas asaccharolytica strain F2A]
ATGAATGTTTCTTTAATGCGTTTGAGTATAGCTTTATTAAGTATGGCTATTCCATCAATGTTATATGCTGCTAATTTAAA
AGATATGGATGTTGTGTCTTTACCTGGTGAACGGGTAGAACTTAAATTAGTGTTTGATGAACCAGTGACGGCTCCAAAGG
GTTATACAATTGATCAGCCGGCACGTATTGCGCTTGATTTGCCAGGTGTTTCGAGCCTACTAGGTGAAAAAAGGAGAACT
GTTGGAATCGGCAATGTTAGAAATCTAACAGTTGTTGAAGCTAAAAATGTAACACGATTGGTAGTAGGTTTAAATACATT
AGTGCCTTACACAACTCGTGCAGAGGGTAATAATATTTTTATTGTATTGGGCGATAGTGTTTCAACTTCACCAATAGTTC
CAACAAATACAGTATCTACATTGCCTACTAAATCGACAAATACTGTTACACCAGTTACTACAAAATCTGTTGCATCTATT
GGAAATGCTATTGAGAATATTGATTTCAGACGTGGTGATAATGGTGAAGGTAATATTGTTGTTAAGCTTTCAAACAGTAC
AATTAGTCCTGATGTACAAGAAAGGGGAGGTAAAATAGTTCTTAATTTCCCTAAAACACAATTACCTGAGTCATTGCGTG
TACGTTTAGATGTTAAGGATTTCGCTACACCAGTACAATTTATTAACTCAACAGCTAAATCAGATGGTTCTAGTATTACT
ATTGAACCTAAAGGTAACTATGACTATTTAGTTTTCCAAGCTAATGATGAATTGACGGTTAGTGTCAAGCATGTATCTGT
TGAAGAAAAACAGCGTCGTTTAGAGGATCAGGGTATTTTCAAAGGTGATACGCTATCTTTAAATTTCCAAGATATTGAAG
TAAGAAGTGTATTACAAATTTTAGCAGACTTTACTAAATTAAATTTAGTGGCTACTGATTCTGTTAGAGGTAATATTACT
TTACGTTTACAAAATGTACCATGGGATCAGGCTTTAGATATTATCATGCGTACAAGAGGTCTTGCTAAGCGTCAAGAAGG
AAATATTCTTATTATTGCACCTGCTGATGAGTTAGCTGAATCTGCCCGTAGAATGTTTGATGCTGAACAAACACTTAAAG
CAACACAACCTTTACGTCGTGAGATTATCCCAGTTAATTATGCTGATGCAACAAGTATTATTGCATTATTCCAAGCTACT
ATGTCACAGACTAATAGTATTCAAGCTGGTCAAAATGCATCTGGTATGGGTAATTATAGTGCGAATATGACCACCAATGA
TCGAGGCTCTTTAACTGTTGATAAGCGTACTAACACTATTATTGCTTATCAAACAGAAGAGCGCTTAGAAGAGTTAAGGC
GTATTATTGCTCAGTTAGATCGTCCTGTGCGTCAAGTACAAATTGAAGCCCGTATTGTTGAGGCCAATGTTGATTTTGCA
AAAAATTTAGGTGTTCGATGGGGAGCAGATTTTGGCTCAAAATCAGGTAATTGGCAAAGTTATGGTGGTGCTGATGGTCG
TAAAAATTCGACAGGTTCTAGTACAGAGTTTGGTAATCTTGGTACAATGTTTACTAATGATGGTGATGGTTATATTCGTA
CACTTGCAGCGCCAGTAGTTGATTTAGGTGCAGCAGATGCTACTTCTGGTTTTGGTATTGGCTTTTTGAGTTCTAATGTG
ATGATTGACTTAAAGCTGAATGCTATGCAGAGATCAGGACAAGGTGAAGTAATTTCTCAACCTAAAGTAACTACTTCAGA
TAAAGAGACAGCACGCATTATGAAAGGTCAGGAAGTGCCTTATCAAGAGGCATCTTCAAGTGGTGCTACTTCTACTTCAT
TTAAAGAGGCATTGCTTTCTTTAGAAGTTACACCACAAATTACTCCAGATAATCAAATTATGATGGCAGTTGTTGTAACT
AAAGACTCTGTTAACGAGTCACAGAGATACCAAGGTGTTCCACCTATTAATAAAAATGAAGTTAATGCTAAAGTATTGGT
CTCTGATGGTGAAACTATTGTATTAGGTGGTGTATTCCAAAATGACCAAAGTAAAATCACTGAAAAAGTTCCTTTCTTAG
GTGATTTACCTGTGATGGGACGTTTATTCCGTCGAGATAGCGTATCAAATACTAAACAAGAGTTATTAATTTTTATAACT
CCTCGTATTATTGATAATAAGGCATTTGCTTCGAGATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(381-465)

AMIN domain (AMIN)

(41-122)

Secretin and TonB N terminus short domain (STN)

(308-354)

Bacterial type II and III secretion system protein (Secretin)

(568-725)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.88

100

0.589
  pilQ Acinetobacter baumannii strain A118

38.564

100

0.396
  pilQ Acinetobacter baumannii D1279779

38.564

100

0.396
  comQ Acinetobacter baylyi ADP1

40

96.311

0.385